[原创]AMV 逆转录酶
<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">产品介绍：</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">AMV </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">逆转录酶从</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体; mso-ansi-language: ZH-CN;">鸟类成髓细胞白血病病毒（amv）中分离出来的，它拥有双重活性：1.</span><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">5' > 3'</font></span></b><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">依赖引物的聚合酶活性，可以以</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">或</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">作为模板；</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">2.</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">具有</span><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">3' > 5'Rase H </font></span></b><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">活性，可以降解</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">RNA/DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">杂合体中的</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">，它需要</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">Mg2+</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">或</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">Mn2+</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">激活。它可用于双脱氧法测定</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">或</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">的序列和以短的</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">为模板合成</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">cDNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">。</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">Moloney Murine Leukemia Virus</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">（</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体; mso-ansi-language: ZH-CN;">莫洛尼</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt;"><font face="Times New Roman">(</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体; mso-ansi-language: ZH-CN;">氏</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt;"><font face="Times New Roman">)</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体; mso-ansi-language: ZH-CN;">鼠白血病病毒</span><span lang="EN-US" style="FONT-FAMILY: 宋体; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体;">M-MuLV</span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">）逆转录酶从</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">E.coli</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">菌株提取出来，此菌株含有一个克隆有</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">M-MuLV RT</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">基因的质粒，</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">M-MuLV</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">基因被删去了</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">RNAase H </font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">核心部分。</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">M-MuLV Rtase</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">是一个依赖</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">的</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">聚合酶，在引物存在的条件下，可以以单链的</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">RNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">或</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">为模板合成互补</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">链，它缺少</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">RNAse H </font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">活性。</span><span lang="EN-US" style="COLOR: black;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">应用：</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><font face="Times New Roman"><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;">1.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;">RT-PCR </span></font><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">和</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">cDNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">合成；</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><font face="Times New Roman"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;">2.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;">RNA</span></font><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">测序和双脱氧法测</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">序列；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">3.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">引物延伸和</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">片段</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">3`-</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-size: 10.5pt;">末端的标记。</span><a name="mulv"></a><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">4</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">逆转录和合成</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">cDNA</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">的第一条链；</span><span lang="EN-US" style="COLOR: black;"><p></p></span></p>
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