[原创]Microcystin微囊藻毒素及其试剂盒、抗体（国内/进口）~~伊普瑞斯科技长期供应
<div class="t_msgfont" id="postmessage_57774">微囊藻毒素Microcystin<br/><br/><br/>Microcystin-LR 微囊藻毒素LR<br/>Microcystin-LR 微囊藻毒素LR<br/>Microcystin-LR 微囊藻毒素LR <br/>MAb to Microcystin-LR (MC10E7) <br/>Microcystin-RR 微囊藻毒素RR<br/>Microcystin-RR 微囊藻毒素RR<br/>Microcystin-RR 微囊藻毒素RR<br/>Microcystin-YR 微囊藻毒素YR<br/>Microcystin-YR 微囊藻毒素YR<br/>Microcystin-LW 微囊藻毒素LW<br/>Microcystin-LW 微囊藻毒素LW<br/>Microcystin-LF 微囊藻毒素LF<br/>Microcystin-LF 微囊藻毒素LF<br/>Nodularin节球藻毒素<br/>Nodularin节球藻毒素<br/>Nodularin节球藻毒素<br/>Nodularin节球藻毒素<br/>Microcystin-LA 微囊藻毒素LA<br/>Microcystin-LA 微囊藻毒素LA<br/>Microcystin-LY 微囊藻毒素LY<br/>Microcystin-LY 微囊藻毒素LY<br/>Monoclonal antibody to Microcystin<br/>Microcystin（Add specific）Elisa kit （欧洲）<br/>Microcystin Elisa kit （美国）<br/>Microcystin Elisa kit （国产）<br/>Cylindrospermopsin柱孢藻毒素<br/>Cylindrospermopsin柱孢藻毒素<br/>Dinophysistoxin-1 鳍藻毒素<br/>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br/>Monoclonal Antibody to Microcystin-LR (MC10E7)<br/><br/>Product Specification <br/><br/>CLONE: MC10E7 <br/><span class="t_tag" href="tag.php?name=ISO">ISO</span>TYPE: Mouse IgG1 <br/>PURITY DETAIL: Protein G-affinity purified. <br/>FORMULATION: Lyophilized. <br/>RECONSTITUTION: Reconstitute with 200μl PBS containing sodium azide. <br/>IMMUNOGEN: Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin. <br/>SPECIFICITY: Recognizes all 4-Arg microcystins. <br/>APPLICATION: ELISA (1:10'000-1:100'000)<br/>Microcystin concentrations can be measured in the range of ~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1'000 ti<span class="t_tag" href="tag.php?name=mes">mes</span> higher compared to UV detection. <br/>SHIPPING: SHIPPED ON BLUE ICE <br/>LONG TERM STORAGE: -20°C <br/>USE/STABILITY: Storage at 4°C will decrease antibody activity and must be avoided. <br/>HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. <br/><br/>Product Description <br/><br/>With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06μg/l have been obtained. The provisional guideline value proposed by the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.<br/>The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1μg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water. <br/><br/>Product Specific Literature References <br/><br/>Highly sensitive immunoassay based on a monoclonal antibody specific for microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001)<br/>Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001) Abstract <br/><br/>General Information <br/><br/>Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa. <br/>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br/>微囊藻毒素ELISA试剂盒说明<br/><br/><br/>一、本试剂盒简介<br/> 本试剂盒系采用自行研制的微囊藻毒素-LR (MC-LR)与BSA的偶联物作为包被抗原，采用自制的抗MC-LR单克隆抗体(MC8C10)作为一抗，组成的MC-LR间接竞争ELISA检测试剂盒。<br/> 该试剂盒可用于水中微囊藻毒素-LR的定量检测；其它固体样品(如藻类、底泥、动物组织等)经过提取后，也可采用该试剂盒进行定量检测。<br/> 该试剂盒特异性好，与第四位非精氨酸的微囊藻毒素几乎没有交叉反应；与第四位为精氨酸的微囊藻毒素有较小的交叉反应(≤10%)。<br/> 敏感性高：最低检测限0.1μg/L；定量检测区间0.2 ~4.0μg/L。<br/> 稳定性强：变异系数≤10%。<br/> 检测时间：~2h<br/> 适应环境温度：20~40oC<br/> 二、试剂盒组成<br/> 1) 使用说明书 1份<br/> 2) 96孔酶标板板 1块 (8孔/条 × 12条) (包被有MC-LR-BSA)<br/> 3) MC-LR标准品 5瓶 (2mL/瓶) (0.2μg/L, 0.5μg/L, 1.0μg/L, 2.0μg/L, 4.0μg/L)<br/> 4) 微囊藻毒素-LR抗体 1瓶 (7mL)<br/> 5) 酶标二抗溶液 1瓶 (12mL)<br/> 6) 显色底物液A 1瓶 (6mL)<br/> 7) 显色底物液B 1瓶 (6mL)<br/> 8) 终止液 1瓶 (7mL)<br/> 9) 浓缩洗涤液(20×) 1瓶 (18mL)<br/> 三、所需仪器和<span class="t_tag" href="tag.php?name=%C9%E8%B1%B8">设备</span><br/> 1) 单道微量移液器(20~200μL) 1支 可选配多道微量移液器(20~200μL) 1支<br/> 2) 酶标仪(含450nm波长)，自带打印功能，或配置打印机，或配置电脑<br/> 3) 0.45μm微滤膜过滤装置，或高速离心机<br/> 4) 4oC冷藏冰箱<br/> 5) 计时器；烧杯等<br/>四、操作流程<br/>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br/><br/> 如有更多其它试剂产品需求与查询,请及时接洽：<a href="mailto:info@express-cn.com">info@express-cn.com</a> 或在我公司查询主页客户留言处（<a href="http://www.express-cn.com/expressgb/lyb.htm" target="_blank">http://www.express-cn.com/expressgb/lyb.htm</a>）留下您的查询内容，我们即会在当天为您作出回复。<br/><br/>伊普瑞斯科技有限公司<br/><a href="mailto:info@express-cn.com">info@express-cn.com</a><br/>总机：010-60206266<br/>传真：010-60206773<br/><a href="http://www.express-cn.com/" target="_blank">www.express-cn.com</a></div>
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