[讨论]传统PCR 扩增Vs .基因合成，您选择谁？
<p class="MsoNormal"><op></op><op></op></p><p class="MsoNormal" style="TEXT-INDENT: 21pt;"><span style="FONT-FAMILY: 宋体;">目前，科研工作者在获取基因序列是最为常用的方法是从</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">文库或组织细胞</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">中通过</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 宋体;">扩增获取有用的</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 宋体;">片段，不过，<span>传统的</span></span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">文库</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 宋体;">扩增</span><span style="FONT-FAMILY: 宋体;">也有着以下明显的缺陷：首先，由于</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">文库一般是由某类或某几类组织制备的，其基因表达有一定的组织特异性，很多基因的表达丰度也相当低，不易钓取，对于这部分基因，即使不算组织获取和</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">文库建立的成本，一些基因钓取的成本也远远高于全长合成的成本。其次，即使成功获得</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 宋体;">产物，产物中也经常含有突变位点或单核苷酸多态性（</span><span lang="EN-US">SNP</span><span style="FONT-FAMILY: 宋体;">），这样的产物在表达和后续试验方面都可能遇到意想不到的问题。</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt;"><span style="FONT-FAMILY: 宋体;">相对</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 宋体;">出发的目标基因扩增方法，全基因合成则并不存在以上问题。由于序列可以根据客户的需要任意定制合成，不会受到目标模板过低、自然存在序列多态</span><span style="FONT-FAMILY: 宋体;">性的影响。同时还可以在序列设计时根据实验目的引入合适密码子偏好，甚至人为引入目标突变位点，或合成自然界中根本不存在的序列以方便研究推进。</span></p><p class="MsoNormal"><b><span lang="EN-US"><op></op><op></op></span></b></p><p class="MsoNormal"><b><span style="FONT-FAMILY: 宋体;">在获取以下类型的基因时，全基因合成比传统</span><span lang="EN-US">PCR</span></b><b><span style="FONT-FAMILY: 宋体;">扩增更有优势</span></b><b><span style="FONT-FAMILY: 宋体;">：</span><span lang="EN-US"><op></op><op></op></span></b></p><p class="MsoNormal" style="TEXT-INDENT: 21pt;"><span lang="EN-US"><op></op><op></op></span></p><p class="MsoNormal"><span class="blacktext"><span style="FONT-FAMILY: 宋体;">·</span><span lang="EN-US"> </span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">组织样本不易获得，但序列已知的基因</span></span></p><p class="MsoNormal"><span class="blacktext"><span style="FONT-FAMILY: 宋体;">·</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">需要特定突变，</span></span><span style="FONT-FAMILY: 宋体;">使用一般的突变技术无法实现<span class="blacktext">的基因</span></span><span class="blacktext"><span lang="EN-US"> <op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><span style="FONT-FAMILY: 宋体;">·</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">采用传统钓取法时间过长，影响实验进程的基因</span></span><span class="blacktext"><span lang="EN-US"> <op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><span style="FONT-FAMILY: 宋体;">·</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">序列保真度要求高，无突变或</span></span><span class="blackenglishtaxt"><span lang="EN-US">SNP</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">存在的基因</span></span><br/> <span class="blacktext"><span lang="EN-US"> <op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><span style="FONT-FAMILY: 宋体;">·</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">需要进行密码子优化，以提高外源表达水平的基因</span></span><span class="blacktext"><span lang="EN-US"> <op></op><op></op></span></span></p><p class="MsoNormal"><span class="blackenglishtaxt"><span lang="EN-US"> </span></span><span class="blackenglishtaxt"><span style="FONT-FAMILY: 宋体;">·</span><span lang="EN-US">cDNA</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">文库中不表达，或表达丰度极低，不易</span></span><span class="blackenglishtaxt"><span lang="EN-US">PCR</span></span><span class="blacktext"><span style="FONT-FAMILY: 宋体;">获取或获取费用较高的基因</span><span lang="EN-US"><op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><b><span style="FONT-FAMILY: 宋体;">金思特全基因合成为您提供更多：</span><span lang="EN-US"><op></op><op></op></span></b></span></p><p class="MsoNormal"><span class="blacktext"><b><span lang="EN-US"><op></op><op></op></span></b></span></p><p class="MsoNormal"><span lang="EN-US">•</span><span style="FONT-FAMILY: 宋体;">克隆任何基因到任何载体：免费克隆至常规</span><span lang="EN-US">T</span><span style="FONT-FAMILY: 宋体;">载体，也可以根据需要克隆到任何商业载体。</span><span lang="EN-US"><br/><br/>•</span><span style="FONT-FAMILY: 宋体;">为您降低成本：为帮助科研人员节省科研成本，全基因合成价格降至</span><span lang="EN-US">4.99</span><span style="FONT-FAMILY: 宋体;">元</span><span lang="EN-US">/bp</span><span style="FONT-FAMILY: 宋体;">。</span><span lang="EN-US"><br/> <br/><br/>•</span><span style="FONT-FAMILY: 宋体;">免费密码子优化：先进的密码子优化工具能极大提高异源宿主中蛋白的表达水平。</span><span lang="EN-US"><br/> <br/><br/>•</span><span style="FONT-FAMILY: 宋体;">客户序列信息严格保密：如果需要我们可以和您签订保密协议。</span></p><p class="MsoNormal"><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><div align="center"><table class="MsoTableGrid" cellspacing="0" cellpadding="0" border="1" style="BORDER-RIGHT: medium none; BORDER-TOP: medium none; BORDER-LEFT: medium none; BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse;"><tbody><tr><td width="189" style="BORDER-RIGHT: black 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: black 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: black 1pt solid; WIDTH: 142pt; PADDING-TOP: 0cm; BORDER-BOTTOM: black 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><b><span style="FONT-FAMILY: 宋体;">基因大小</span></b><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td><td width="189" style="BORDER-RIGHT: black 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: black 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: black 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><b><span style="FONT-FAMILY: 宋体;">价格</span></b><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td><td width="189" style="BORDER-RIGHT: black 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: black 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: black 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><b><span style="FONT-FAMILY: 宋体;">交货时间</span></b><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td></tr><tr><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: 1pt solid; WIDTH: 142pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span style="FONT-FAMILY: 宋体;">小于<span lang="EN-US">100bp</span></span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;"><op></op><op></op></span></p><br/> </td><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span style="FONT-FAMILY: 宋体;">￥<span lang="EN-US">499.00/</span>条</span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;"><op></op><op></op></span></p><br/> </td><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US" style="FONT-FAMILY: 宋体;">8</span><span style="FONT-FAMILY: 宋体;">个工作日左右<span lang="EN-US"><op></op><op></op></span></span></p><br/> </td></tr><tr><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: 1pt solid; WIDTH: 142pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US" style="FONT-FAMILY: 宋体;">100-1000bp</span><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td><td width="189" rowspan="2" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><b><span style="FONT-FAMILY: 宋体;">￥</span><span lang="EN-US">4.99</span></b><span lang="EN-US">/<b>bp</b><span class="blacktext"><op></op><op></op></span></span></p><br/> </td><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US" style="FONT-FAMILY: 宋体;">10</span><span style="FONT-FAMILY: 宋体;">个工作日左右</span><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td></tr><tr><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: 1pt solid; WIDTH: 142pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US" style="FONT-FAMILY: 宋体;">1000-3000bp</span><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td><td width="189" style="BORDER-RIGHT: 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 142.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: 1pt solid;"><br/> <p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US" style="FONT-FAMILY: 宋体;">15</span><span style="FONT-FAMILY: 宋体;">个工作日左右</span><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/> </td></tr></tbody></table></div><p class="MsoNormal" align="center" style="TEXT-ALIGN: center;"><span lang="EN-US">*</span><span style="FONT-FAMILY: 宋体;">此价格仅适用于非复杂序列的基因合成，复杂序列基因合成请询价。</span><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><p class="MsoNormal"><span class="blacktext"><span lang="EN-US"><op></op><op></op></span></span></p><br/><br/><br/><br/><p class="MsoNormal" align="left" style="TEXT-ALIGN: left;"><span style="COLOR: black; FONT-FAMILY: 宋体;">详情请登陆：</span><span lang="EN-US"><a href="http://www.jinsite.com.cn/gene_synthesis.html">http://www.jinsite.com.cn/gene_synthesis.html</a></span><br/><span style="COLOR: black; FONT-FAMILY: 宋体;">金思特科技（南京）有限公司<span lang="EN-US"><br/></span></span><span style="FONT-FAMILY: 宋体;">贵宾热线：</span><span lang="EN-US">025-84347333<br/></span><span style="FONT-FAMILY: 宋体;">订购邮箱：</span><span lang="EN-US">gene@jinsite.com.cn</span></p><br/><br/>
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