[原创]SYBR绿色核酸凝胶染液
<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 15pt; FONT-FAMILY: 黑体;"><p class="MsoNormal" align="center" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: center;"><span>SYBR绿色核酸凝胶染液 <p></p></span></p><p></p><p></p><p class="MsoNormal" align="center" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: center;"><font size="3"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">（</span><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt;">SYBR Green Nucleic Acid Gel Stains</span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">）</span><span lang="EN-US" style="FONT-SIZE: 14pt;"><p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman" size="3"> </font></p></span></p><p><font face="Times New Roman" size="3"> </font></p><font size="3"><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">应用</span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">：</span></font>
<p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 100.95pt; mso-char-indent-count: 8.38;"><span>DNA<font size="3">和</font>RNA<font size="3">的检测；<span lang="EN-US"><span style="mso-spacerun: yes;"> </span></span></font>SSCP<font size="3">及异源双链分析。<span lang="EN-US">
<p></p></span></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">备注：</span><span lang="EN-US" style="FONT-SIZE: 12pt;">
<p></p></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 21pt; TEXT-INDENT: -21pt; mso-list: l0 level1 lfo1; tab-stops: list 21.0pt;"><span><span style="mso-list: Ignore;"><font size="3">Ø</font><span style="FONT: 7pt 'Times New Roman';"> </span></span>SYBR</span><font size="3"><span style="COLOR: fuchsia; FONT-FAMILY: 宋体;">绿色<span lang="EN-US">I核酸凝胶染液</span></span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">（</span><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: fuchsia; FONT-FAMILY: 黑体;">SYBR Green I Nucleic Acid Gel Stains</span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">）</span><span lang="EN-US">
<p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24.1pt; mso-char-indent-count: 2.0;"><span>SYBR<font size="3">绿色核酸凝胶染液（</font></span><font size="3"><span lang="EN-US" style="COLOR: fuchsia; FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt;">SYBR Green Nucleic Acid Gel Stains</span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">）</span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">是一种高敏感性的荧光染液，用于检测琼脂糖凝胶及聚丙烯酰胺凝胶中的双链</span><span lang="EN-US"><font face="Times New Roman">DNA</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">和寡核苷酸。当染液与核酸结合后</span><span lang="EN-US"><font face="Times New Roman">SYBR</font></span></font><span><font size="3">绿色核酸凝胶染液的荧光信号会明显增强，因此经</font>SYBR</span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体;"><font size="3">绿色<span lang="EN-US">I核酸凝胶染液</span></font></span><span><font size="3">染色的凝胶显示出非常好的信躁比，没有背景荧光。为获得最佳的结果，凝胶最好在跑胶后再进行染色。</font>SYBR</span><font size="3"><span style="COLOR: fuchsia; FONT-FAMILY: 宋体;">绿色<span lang="EN-US">I核酸凝胶染液</span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">用于<u><span style="COLOR: blue;">低含量的</span></u></span><u><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: blue; FONT-FAMILY: 黑体;">PCR</span><span style="COLOR: blue; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">产物，凋亡研究及异源双链分析中少量</span><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: blue; FONT-FAMILY: 黑体;">DNA</span></u><u><span style="COLOR: blue; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">的检测</span></u><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">较为理想。</span><span lang="EN-US">
<p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 21pt; TEXT-INDENT: -21pt; mso-list: l0 level1 lfo1; tab-stops: list 21.0pt;"><span><span style="mso-list: Ignore;">Ø<span style="FONT: 7pt 'Times New Roman';"> </span></span>SYBR</span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体;"><font size="3">绿色<span lang="EN-US">II核酸凝胶染液</span></font></span><span style="FONT-SIZE: 12pt; COLOR: fuchsia; FONT-FAMILY: 黑体;">（<span lang="EN-US">SYBR Green<span style="mso-spacerun: yes;"> </span>II Nucleic Acid Gel Stains）</span></span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">
<p></p></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 21pt; mso-char-indent-count: 2.0;"><font size="3"><span style="FONT-FAMILY: 宋体;">在</span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 黑体; mso-hansi-font-family: 宋体;">ssDNA</span><span style="FONT-FAMILY: 宋体;">及</span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 黑体; mso-hansi-font-family: 宋体;">RNA</span></font><span><font size="3">分析中，</font>SYBR</span><font size="3"><span style="COLOR: fuchsia; FONT-FAMILY: 宋体;">绿色<span lang="EN-US">II核酸凝胶染液</span></span><u><span style="COLOR: blue; FONT-FAMILY: 宋体;">具有</span><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: blue; FONT-FAMILY: 黑体; mso-hansi-font-family: 宋体;">SYBR</span></u><u><span style="COLOR: blue; FONT-FAMILY: 宋体;">绿色<span lang="EN-US">I核酸凝胶染液所具有的所有优点</span></span></u><span style="FONT-FAMILY: 宋体;">，并且适用于<u><span lang="EN-US" style="COLOR: blue;">DNA非等位基因缺失突变的</span></u></span><u><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: blue; FONT-FAMILY: 黑体; mso-hansi-font-family: 宋体;">SSCP</span><span style="COLOR: blue; FONT-FAMILY: 宋体;">分析</span></u><span style="FONT-FAMILY: 宋体;">。</span><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">
<p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 21pt; TEXT-INDENT: -21pt; mso-list: l0 level1 lfo1; tab-stops: list 21.0pt;"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: Wingdings; mso-fareast-font-family: Wingdings; mso-bidi-font-family: Wingdings;"><span style="mso-list: Ignore;">Ø<span style="FONT: 7pt 'Times New Roman';"> </span></span></span><shapetype id="_x0000_t75" stroked="f" filled="f" path="m@4@5l@4@11@9@11@9@5xe" coordsize="21600,21600" ospt="75" opreferrelative="t"></shapetype><stroke joinstyle="miter"></stroke><stroke></stroke><formulas></formulas><f eqn="if lineDrawn pixelLineWidth 0"></f><f></f><f eqn="sum @0 1 0"></f><f></f><f eqn="sum 0 0 @1"></f><f></f><f eqn="prod @2 1 2"></f><f></f><f eqn="prod @3 21600 pixelWidth"></f><f></f><f eqn="prod @3 21600 pixelHeight"></f><f></f><f eqn="sum @0 0 1"></f><f></f><f eqn="prod @6 1 2"></f><f></f><f eqn="prod @7 21600 pixelWidth"></f><f></f><f eqn="sum @8 21600 0"></f><f></f><f eqn="prod @7 21600 pixelHeight"></f><f></f><f eqn="sum @10 21600 0"></f><f></f><formulas></formulas><path gradientshapeok="t" oextrusionok="f" oconnecttype="rect"></path><path></path><lock aspectratio="t" vext="edit"></lock><lock></lock><shapetype></shapetype><shape id="_x0000_s1026" type="#_x0000_t75" style="MARGIN-TOP: 43.3pt; Z-INDEX: -1; LEFT: 0px; MARGIN-LEFT: 261pt; WIDTH: 180pt; POSITION: absolute; HEIGHT: 180pt; TEXT-ALIGN: left;"></shape><imagedata src="file:///C:\DOCUME~1\lnq\LOCALS~1\Temp\msohtml1\01\clip_image001.jpg" otitle="SYBR"></imagedata><imagedata></imagedata><shape></shape><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: fuchsia; FONT-FAMILY: 黑体;">SYBR</span><font size="3"><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">绿色凝胶染液照相滤光片（</span><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: fuchsia; FONT-FAMILY: 黑体;">SYBR Green Gel Stain Photographic Filter</span><span style="COLOR: fuchsia; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">）</span></font><span><font size="3">为使黑色摄像获得最佳的检测敏感性，经</font>SYBR</span><font size="3"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">绿色凝胶染色的凝胶应使用</span><font face="Times New Roman"><br/> </font></font><span><font size="3">一种</font>SYBR</span><span><font size="3">绿色照相滤片进行摄影。这种滤光片适用于大多数</font>Polaroid</span><font size="3"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">相机系列。</span><span lang="EN-US" style="FONT-SIZE: 12pt;">
<p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 12pt;"><p><font face="Times New Roman"> </font></p></span></p><p><font face="Times New Roman"> </font></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 21pt; TEXT-INDENT: -21pt; mso-list: l0 level1 lfo1; tab-stops: list 21.0pt;"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: Wingdings; mso-fareast-font-family: Wingdings; mso-bidi-font-family: Wingdings;"><span style="mso-list: Ignore;">Ø<span style="FONT: 7pt 'Times New Roman';"> </span></span></span><font size="3"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体;">如有需要了解产品的详细信息，可以邮件、电话联系我们。</span><span lang="EN-US" style="FONT-SIZE: 12pt;">
<p></p></span></font></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">相关信息如下：</span><span lang="EN-US" style="FONT-SIZE: 12pt;">
<p></p></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 12pt;"><p><font face="Times New Roman"> </font></p></span></p><p><font face="Times New Roman"> </font></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: fuchsia; FONT-FAMILY: 黑体;">SYBR Green I Nucleic Acid Gel Stains</span><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><font size="3"><br/> <p></p></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><font size="3">Detection Limits <p></p></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><font size="3"><span style="mso-spacerun: yes;"> </span>々<span style="mso-spacerun: yes;"> </span>dsDNA at 300nm transillumination: 60 pg <p></p></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><font size="3"><span style="mso-spacerun: yes;"> </span>々<span style="mso-spacerun: yes;"> </span>Oligonucleotides at 300nm <p></p></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><font size="3">transillumination 1-2 ng <p></p></font></span></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span lang="EN-US" style="FONT-FAMILY: 黑体; mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 宋体;"><p><font size="3"> </font></p></span></p><p><font size="3"> </font></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.25pt; TEXT-INDENT: -17.25pt; mso-char-indent-count: -1.5;"><span style="FONT-SIZE: 11.5pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial;">毕特博生物技术有限责任公司</span><span style="FONT-SIZE: 11.5pt; FONT-FAMILY: Arial; mso-font-kerning: 0pt;"><br/> <span lang="EN-US">
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[此贴子已经被作者于2008-11-28 21:58:38编辑过]
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