[分享]β淀粉样蛋白(βAmyloid)ELISA检测样本前处理方法，仅供参考！
<p class="MsoNormal" align="left" style="BACKGROUND: #f7f9fd; MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 14.4pt; TEXT-ALIGN: left;"><span><font style="BACKGROUND-COLOR: #ffffff;">β淀粉样蛋白(βAmyloid)ELISA检测样本前处理方法<br/>样品前处理<br/>◆脑组织<br/>1. 聚集的Aβ（阿滋海默症病人的脑组织）<br/>①1g脑组织切碎后加5倍体积的TBS，用均质器均质（10次）。 组织匀浆500000×g，4℃离心20分钟。<br/>TBS：50 mmol/L Tris­HCl , pH 7.6, 150 mmol/L NaCl , 蛋白酶抑制剂 <br/>②沉淀物悬浮与5倍体积的TBA/蛋白酶抑制剂（含1 mol/L蔗糖），500000×g，4℃离心20分钟。<br/>③沉淀物中加入3倍体积的1％Triton X­100/TBS/蛋白酶抑制剂，匀质（10次）。37℃孵育15分钟后，500000×g离心20分钟。<br/>④沉淀物和匀质物中加入3倍体积的2％SDS/TBS/蛋白酶抑制剂到。组织匀浆37℃孵育15分钟后，500000×g 25℃离心。<br/>⑤沉淀物中加1mL 70％蚁酸到，超声破碎后500000×g 4℃离心<br/>⑥收集上清液并用离心浓缩(Speed Vac)干燥。加100μL DMSO并短时超声破碎成悬液。零下80℃储存这个DMSO－蚁酸溶解的Aβ溶液直到使用。<br/>⑦检测时，依照说明书所写，用试剂盒中的标准稀释液溶解样本。推荐稀释度是Aβ40稀释1,000倍，Aβ42稀释100倍。<br/>2. 可溶Aβ（正常脑组织）<br/>2-1. 使用70％蚁酸<br/>①150mg组织加入1mL 70％蚁酸中匀质(敲6次)后100,000×g离心1小时。<br/>②用1 mol/L Tris二十倍稀释上清液<br/>③用试剂盒中的标准稀释液稀释样品后，按照说明书所述方法检测。<br/>2-2. 使用5.0 mol/L盐酸胍<br/>①组织加入10倍体积预冷的L盐酸胍溶液（5.0 mol/L / 50 mmol/L Tris­Cl, pH8.0）后匀质，室温下3～4小时。<br/>②组织匀浆中加入10倍体积的预冷酪蛋白缓冲剂（0.25%干酪素/ 0.05% 叠氮化钠/ 20μg/ml抑肽酶/ 5 mmol/l EDTA, pH8.0 / 10μg/mL亮肽素，溶于PBS），16,000×g，4℃离心20分钟。 <br/>③用试剂盒中的标准稀释液稀释上清液后，按照说明书所述方法检测。<br/>◆细胞培养上清液<br/>根据需要用试剂盒中的标准稀释液稀释样品，然后按照说明书所述方法检测。推荐稀释浓度是Aβ40稀释5倍，Aβ42无需稀释。<br/>◆ 脑脊液<br/>根据需要用试剂盒中的标准稀释液稀释样品，然后按照说明书所述方法检测。推荐稀释浓度是50倍。<br/>◆ 血浆<br/>①用EDTA2K离心收集在真空血液收集管中的血液，5,000×g，15分钟，4℃，分离血浆样品，然后储存在零下80℃直到使用。<br/>②用试剂盒中的标准稀释液4倍稀释血浆以避免血浆中的干扰物质影响检测，按照说明书指定方法检测。</font></span></p><p class="MsoNormal" align="left" style="BACKGROUND: #f7f9fd; MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 14.4pt; TEXT-ALIGN: left;"><span><font style="BACKGROUND-COLOR: #ffffff;">上海西宝生物科技有限公司 <br/>地址:上海张江高科技园区毕升路299号11-502# 邮编: 201204<br/>电话: 021-5027 2975/76/77/78转6216<br/>联系人：金育华 13817370085<br/>http: //www.cxbio.com E-mail:market@cxbio.com<br/>http: //www.cnbio.net ___中国生物网</font></span></p><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span><p></p>[此贴子已经被作者于2008-12-11 16:15:22编辑过] <p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;"><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">c沉淀物中加入</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">3</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">倍体积的</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">1</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">％</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">Triton X­100/TBS/</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">蛋白酶抑制剂，匀质（</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">10</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">次）。</span><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="37" unitname="℃"><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">37</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">℃</span></chmetcnv><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">孵育</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">15</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">分钟后，</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">500000</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">×</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">g</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">离心</span><span lang="EN-US" style="FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; mso-fareast-font-family: 宋体; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">20</span><span style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">分钟。</span>④沉淀物和匀质物中加入</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">3</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">倍体积的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">2</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">％</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">SDS/TBS/</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">蛋白酶抑制剂到。组织匀浆</span><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="37" unitname="℃"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">37</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">孵育</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">15</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">分钟后，</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">500000</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">×</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g </font><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="25" unitname="℃"><font face="Times New Roman">25</font><span lang="EN-US" style="FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";"><span lang="EN-US">℃</span></span></chmetcnv><span lang="EN-US" style="FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">离心。</span><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">d沉淀物中加</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">1mL 70</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">％蚁酸到，超声破碎后</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">500000</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">×</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g </font><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="4" unitname="℃"><font face="Times New Roman">4</font><span lang="EN-US" style="FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";"><span lang="EN-US">℃</span></span></chmetcnv><span lang="EN-US" style="FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">离心</span><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">e收集上清液并用离心浓缩</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">(Speed Vac)</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">干燥。加</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">100</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">μ</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">L DMSO</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">并短时超声破碎成悬液。<chmetcnv wst="on" tcsc="0" numbertype="1" negative="True" hasspace="False" sourcevalue="80" unitname="℃">零下<span lang="EN-US" style="FONT-FAMILY: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">80</span>℃</chmetcnv>储存这个</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">DMSO</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">－蚁酸溶解的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">A</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">β溶液直到使用</span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">f检测时，依照试剂盒要求，使用标准稀释液溶解样本.。</span></p> <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">2. </font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">可溶</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">A</font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">β(正常脑组织)</span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">2-1. </font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">使用</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">70</font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">％蚁酸</span></b><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">①</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">150mg</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">组织加入</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">1mL 70</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">％蚁酸中匀质</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">(</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">敲</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">6</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">次</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">)</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">后</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">100,000</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">×</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">离心</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">1</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">小时。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">②用</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">1 mol/L Tris</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">二十倍稀释上清液</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 17.8pt; TEXT-INDENT: -8.9pt; mso-para-margin-left: .85gd; mso-char-indent-count: -.85;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">③用试剂盒中的标准稀释液稀释样品后，按照说明书所述方法检测。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p> <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">2-2. </font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">使用</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">5.0 mol/L</font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">盐酸胍</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 10.5pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-char-indent-count: 1.0;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">①组织加入</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">10</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">倍体积预冷的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">L</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">盐酸胍溶液（</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">5.0 mol/L / 50 mmol/L Tris­Cl, pH8.0</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">）后匀质，室温下</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">3</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">～</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">4</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">小时。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 8.9pt; TEXT-INDENT: 1.45pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-para-margin-left: .85gd; mso-char-indent-count: .14;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">②组织匀浆中加入</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">10</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">倍体积的预冷酪蛋白缓冲剂（</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">0.25%</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">干酪素</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">/ <span style="mso-spacerun: yes;"> </span>0.05% </font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">叠氮化钠</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">/ 20</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">μ</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g/ml</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">抑肽酶</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">/ 5 mmol/l EDTA, pH8.0 / 10</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">μ</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g/mL</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">亮肽素，溶于</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">PBS</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">），</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">16,000</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">×</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">，</span><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="4" unitname="℃"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">4</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">离心</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">20</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">分钟。</span><span style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">
<span lang="EN-US"><p></p></span></font></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 10.5pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-char-indent-count: 1.0;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">③用试剂盒中的标准稀释液稀释上清液后，按照说明书所述方法检测。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p> <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">◆细胞培养上清液</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">根据需要用试剂盒中的标准稀释液稀释样品，然后按照说明书所述方法检测。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">A</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">β</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">40</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: "Courier New";">浓度是</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">A</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">β</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">42</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">5</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">倍</span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: "Courier New";">。</span><span lang="EN-US" style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: "Courier New";"><p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-FAMILY: 新宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 新宋体;"><span style="mso-list: Ignore;">◆<span style="FONT: 7pt "Times New Roman";"> </span></span></span><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">脑脊液</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">根据需要用试剂盒中的标准稀释液稀释样品，然后按照说明书所述方法检测。</span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: "Courier New";">按试剂盒要求稀释。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p> <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-FAMILY: 新宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 新宋体;"><span style="mso-list: Ignore;">◆<span style="FONT: 7pt "Times New Roman";"> </span></span></span><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">血浆</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 10.5pt; TEXT-ALIGN: left; mso-layout-grid-align: none; mso-char-indent-count: 1.0;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">①用</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">EDTA2K</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">离心收集在真空血液收集管中的血液，</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">5,000</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">×</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">g</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">，</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">15</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">分钟，</span><chmetcnv wst="on" tcsc="0" numbertype="1" negative="False" hasspace="False" sourcevalue="4" unitname="℃"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><font face="Times New Roman">4</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">，分离血浆样品，然后储存在<chmetcnv wst="on" tcsc="0" numbertype="1" negative="True" hasspace="False" sourcevalue="80" unitname="℃">零下<span lang="EN-US" style="FONT-FAMILY: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">80</span>℃</chmetcnv>直到使用。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p><p class="MsoNormal" align="left" style="BACKGROUND: #f7f9fd; MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 14.4pt; TEXT-ALIGN: left;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-font-family: PITT-4-002-00;">②用试剂盒中的标准稀释液稀释血浆以避免血浆中的干扰物质影响检测，按照说明书指定方法检测。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: PITT-4-002-00;"><p></p></span></p>
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