[原创]免疫共沉淀（Protein G-agarose）试剂盒
<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><font face="Times New Roman"><b><span lang="EN-US" style="FONT-SIZE: 16pt; COLOR: black; mso-font-kerning: 0pt;">Immunoprecipitation Kit<span style="mso-spacerun: yes;"> </span></span></b><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: black; mso-font-kerning: 0pt;">Protein G-agarose<p></p></span></font></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span style="FONT-SIZE: 15pt; COLOR: black; FONT-FAMILY: 宋体; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">免疫共沉淀（</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: black; mso-font-kerning: 0pt;"><font face="Times New Roman">Protein G-agarose</font></span><span style="FONT-SIZE: 15pt; COLOR: black; FONT-FAMILY: 宋体; mso-font-kerning: 0pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">）试剂盒</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: black; mso-font-kerning: 0pt;"><p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 16pt; COLOR: black; mso-font-kerning: 0pt;"><p><font face="Times New Roman"> </font></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: black; mso-font-kerning: 0pt;"><font face="Times New Roman">Catalogue #: IPK<chmetcnv wst="on" unitname="g" sourcevalue="1" hasspace="False" negative="False" numbertype="1" tcsc="0">001G</chmetcnv><span style="mso-spacerun: yes;"> </span>Storage: 4</font></span><span style="FONT-SIZE: 11pt; COLOR: black; FONT-FAMILY: 宋体; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体; mso-hansi-font-family: "Times New Roman";">－</span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: black; mso-font-kerning: 0pt;"><font face="Times New Roman">8 ºC<p></p></font></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Description:<p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">The Protein G-agarose Purification Kit is designed for rapid purification of proteins expressed in cultured cells including<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">mammalian cells, insect cells, yeast and E. coli. The easy-to-follow procedure is based on novel protein purification<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">chemistry. Purification may take place under native conditions or under denaturing conditions depending on the<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">solubility and/or desired application of the expressed protein. The purified protein can be used directly for enzymatic<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">assays, protein biochemical analyses, SDS-PAGE, as well as other protein based applications.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Size: </span></b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">30 standard assays<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Kit components:<p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><i><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></i></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><i><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Components Name Cat# Size<p></p></span></i></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Component A Protein G-agarose IR004 1 mL<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Component B Binding Buffer N/A 50 mL<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Component C Washing Buffer (5X) N/A 50 mL<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Component D Elution Buffer N/A 10 mL<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Component E Neutralization Buffer N/A 1 mL<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Reagents needed, but not provided in the kit:<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><font face="Times New Roman"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; mso-font-kerning: 0pt;">􀂗</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Wingdings-Regular; mso-font-kerning: 0pt; mso-bidi-font-family: Wingdings-Regular;"></span></font><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">DTT (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat. #: MC010</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">)<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><font face="Times New Roman"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; mso-font-kerning: 0pt;">􀂗</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Wingdings-Regular; mso-font-kerning: 0pt; mso-bidi-font-family: Wingdings-Regular;">
</span></font><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Phosphate Buffered Saline (PBS) (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat. #: CC008</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">)<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><font face="Times New Roman"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; mso-font-kerning: 0pt;">􀂗</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Wingdings-Regular; mso-font-kerning: 0pt; mso-bidi-font-family: Wingdings-Regular;">
</span></font><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Proteinase Inhibitor Cocktails (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat. #: MP027</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">)<p></p></span></p> <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Precedures:<p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">A. Preparation of Cell Lysates (for adherent mammalian cells)<p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">1. Remove the growth medium from the cells to be analyzed. Rinse the cells twice with PBS buffer (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat# CC008</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">).<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">2. Add 10ml (10-cm plate), scrape the cells off the plate and transfer cells into 15-cm Folcon tube.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">3. Centrifuge the sample at 1000 x g for 5 mins.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">4. Discard the PBS, add lysis buffer (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat# MP011T</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">) supplemented with <chmetcnv wst="on" unitname="mm" sourcevalue="1" hasspace="False" negative="False" numbertype="1" tcsc="0">1mM</chmetcnv> DTT (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat. #: MC010</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">) and Proteinase<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Inhibitor Cocktails (</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: #33339b; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">Cat. #: MP027</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">) (10</span><span lang="EN-US" style="FONT-SIZE: 6pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">6</span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">-10</span><span lang="EN-US" style="FONT-SIZE: 6pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">7 </span><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">cells/mL).<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">5. Incubate the cells for 15-30 minutes on a shaker.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">6. Centrifuge the cell lysate for 10 minutes at 12,000 x g.</span><span lang="EN-US" style="FONT-SIZE: 10pt; COLOR: black; mso-font-kerning: 0pt;"><p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">7. Transfer the supernatant to a 1.5ml eppendorf tube.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">8. For immediate use, keep on ice. If the supernatant is not to be used immediately, store it at –<chmetcnv wst="on" unitname="ﾰC" sourcevalue="70" hasspace="True" negative="False" numbertype="1" tcsc="0">70 °C</chmetcnv>.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><b><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">B. Preparation of Protein A-agarose/antibody Complex and Immunoprecipitation<p></p></span></b></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;"><p> </p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">1. Thoroughly suspend the protein G-agarose beads.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">2. Transfer 30ul of the gel suspension to a 1.5ml eppendorf tube. (For beads transfer, use plastic pipette tip with the<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none;"><span lang="EN-US" style="FONT-SIZE: 9pt; COLOR: black; FONT-FAMILY: Verdana; mso-font-kerning: 0pt; mso-bidi-font-family: Verdana;">end cut for about <chmetcnv wst="on" unitname="mm" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0">2mm</chmetcnv> to allow the beads to be transferred).<p></p></span></p> <p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">迈晨科技（北京）有限公司竭诚为您服务 ！<span lang="EN-US"><p></p></span></span></p><p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">详情请登录网站：<span lang="EN-US">www.macgene.com</span>，联系电话：<span lang="EN-US">010-82057786<p></p></span></span></p> 迈晨科技（北京）有限公司竭诚为您服务 ！
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