[原创]单荧光素酶活性检测试剂盒

macgene 发表于 2009-1-5 09:54:00

uciferase Assay Kit  荧光素酶活性检测试剂盒产品编号：MPK003   储存条件：－20 ºC<span style="FONT-SIZE: 11pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">简介：<span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">迈晨公司开发生产的“荧光素酶活性检测试剂盒”The Luciferase Assay Kit provides a sensitive, quick, and quantitative measurement of the activity of firefly luciferase produced in cultured mammalian cells. The kit contains a cell lysis buffer, an assay buffer, and the luciferase substrate. The cell lysis buffer allows efficient extraction of luciferase from many types of cultured cells within 5 minutes. The luciferase assay is performed by adding the luciferase substrate to the cell lysate premixed with the assay buffer. Luciferase in the cell lysate catalyzes the chemiluminescent reaction, which emits light that is readily measurable with a luminometer. <span style="FONT-SIZE: 11pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">试剂盒组分： <table class="MsoTableGrid" cellspacing="0" cellpadding="0" border="0" style="MARGIN: auto auto auto 27pt; BORDER-COLLAPSE: collapse; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-yfti-tbllook: 480;"><tbody><tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes;"><td valign="top" width="216" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 162pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"><span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">试剂盒提供试剂</td><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Quantity</td><td valign="top" width="324" rowspan="4" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 243pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"> 注：
该试剂盒提供的试剂能够满足100次分析，单次分析是指24孔或者12孔细胞培养板的一孔</td></tr><tr style="mso-yfti-irow: 1;"><td valign="top" width="216" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 162pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"><span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">细胞裂解液（Extraction Buffer<span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">）</td><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">10mL</td></tr><tr style="mso-yfti-irow: 2;"><td valign="top" width="216" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 162pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"><span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">分析试剂（Assay Buffer<span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">）</td><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">30mL</td></tr><tr style="mso-yfti-irow: 3; mso-yfti-lastrow: yes;"><td valign="top" width="216" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 162pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"><span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">荧光素底物（Substrate<span style="FONT-SIZE: 9pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">）</td><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">10mL</td></tr></tbody></table>

macgene 发表于 2009-1-5 09:54:00

<span style="FONT-SIZE: 11pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">操作步骤： <span style="FONT-SIZE: 10pt; COLOR: black; LINE-HEIGHT: 150%; FONT-FAMILY: 宋体; mso-bidi-font-family: Arial-ItalicMT; mso-ascii-font-family: Verdana; mso-hansi-font-family: Verdana; mso-bidi-font-style: italic;">一、细胞裂解<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l1 level1 lfo2; tab-stops: list 18.0pt;">Ø       从细胞培养板中吸去培养液<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l1 level1 lfo2; tab-stops: list 18.0pt;">Ø       每孔中加入100微升细胞裂解液（Extraction Buffer
）<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l1 level1 lfo2; tab-stops: list 18.0pt;">Ø       于室温在摇床上摇动5－10分钟<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l1 level1 lfo2; tab-stops: list 18.0pt;">Ø       将细胞培养板放置在冰上备用 二、荧光强度测定 1. Single-Tube Measurement with Manual Injection <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Program the luminometer to perform a 2-second measurement delay followed by a 10-second measurement read for luciferase activity. The read time may be shortened if sufficient light is produced. <p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Dispense 300µl of the Luciferase Assay Buffer into luminometer tubes, one tube per sample.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Add 90µl of cell lysate to a luminometer tube containing the Luciferase Assay Buffer. Mix by pipetting 2–3 times or vortex briefly.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Add 100µl of Substrate to a luminometer tube containing the Luciferase Assay Buffer and cell lysate. Mix by pipetting 2–3 times or vortex briefly.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Place the tube in the luminometer and initiate reading.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Record the reading.

macgene 发表于 2009-1-5 09:54:00

2. Single-Tube Measurement with Automatic Injection Ø       Program the luminometer to perform a 2-second measurement delay followed by a 10-second measurement read for luciferase activity. Ø       Prime the luminometer injector at least three times with Luciferase Substrate Reagent.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Dispense 300µl of the Luciferase Assay Buffer into luminometer tubes, one tube per sample.Ø       Add 90µl of cell lysate to a luminometer tube containing the Luciferase Assay Buffer. Mix by pipetting 2–3 times or vortex briefly.Ø       Place the tube in the luminometer and initiate reading by injecting 100µl of Substrate into the tube.Ø       Record the reading. 1.      Multi-Tube Measurement with Automatic Injection Ø       Program the luminometer to perform a 2-second measurement delay followed by a 10-second measurement read for luciferase activity. Ø       Program the luminometer with at least three injections with Luciferase Substrate.<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;">Ø       Dispense 300µl of the Luciferase Assay Buffer into luminometer tubes, one tube per sample.Ø       Add 90µl of cell lysate to a luminometer tube containing the Luciferase Assay Buffer. Ø       Place the tubes in the luminometer chain and initiate reading by injecting 100µl of Substrate into the tube.Ø       Record the reading.

macgene 发表于 2009-3-30 12:34:13

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[原创]单 荧光素酶活性检测试剂盒

[原创]单荧光素酶活性检测试剂盒