[原创]逆转录病毒感染试剂盒

macgene 发表于 2009-1-5 09:55:00

<span style="FONT-SIZE: 16pt; FONT-FAMILY: 宋体; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;">Retroviral Infection Kit逆转录病毒感染试剂盒Catalogue：CTK002  Storage: 4－8 ºCKit components: <table class="MsoTableGrid" cellspacing="0" cellpadding="0" border="0" style="BORDER-COLLAPSE: collapse; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-yfti-tbllook: 480;"><tbody><tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Components</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Name</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Size</td></tr><tr style="mso-yfti-irow: 1;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">A</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">HBS</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">100mL</td></tr><tr style="mso-yfti-irow: 2;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">B</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">CaCl2</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">10mL</td></tr><tr style="mso-yfti-irow: 3;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">C</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Helper</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">20ug in 20uL</td></tr><tr style="mso-yfti-irow: 4;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">D</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">pMac-vGFP</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">20ug in 20uL</td></tr><tr style="mso-yfti-irow: 5; mso-yfti-lastrow: yes;"><td valign="top" width="108" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">E</td><td valign="top" width="136" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 102.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">Polybrene</td><td valign="top" width="109" style="BORDER-RIGHT: #ece9d8; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 81.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;">1mL</td></tr></tbody></table>Principle: The MSCV (Murine Stem Cell Virus) Retroviral Expression System contains vectors that are optimized for introducing and expressing target genes in proliferating murine or human cell lines. This highly efficient system is ideal for analyzing gene expression and function in development, embryogenesis, and carcinogenesis in both cell culture and transgenic assays. Designed for tough-to-transfect cells, the MSCV system contains two vectors: pMac-vGFP and helper that expresses protein elements required for producing high-titer virus. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell virus that allows you to transduce most hard-to-transfect cell lines. This system drives high-level, constitutive expression of the target gene in most mammalian cell lines.  <shapetype id="_x0000_t202" path="m,l,21600r21600,l21600,xe" ospt="202" coordsize="21600,21600"><stroke joinstyle="miter"></stroke><path oconnecttype="rect" gradientshapeok="t"></path></shapetype><shape id="_x0000_s1026" stroked="f" type="#_x0000_t202" style="MARGIN-TOP: 2.55pt; Z-INDEX: 1; LEFT: 0px; MARGIN-LEFT: 404.85pt; WIDTH: 134.4pt; POSITION: absolute; HEIGHT: 217.2pt; TEXT-ALIGN: left; mso-wrap-style: none;"><textbox style="mso-fit-shape-to-text: t;"><table cellspacing="0" cellpadding="0" width="100%"><tbody><tr><td style="BORDER-RIGHT: #ece9d8; BORDER-TOP: #ece9d8; BORDER-LEFT: #ece9d8; BORDER-BOTTOM: #ece9d8; BACKGROUND-COLOR: transparent;"><div><shapetype id="_x0000_t75" path="m@4@5l@4@11@9@11@9@5xe" ospt="75" coordsize="21600,21600" stroked="f" filled="f" opreferrelative="t"><stroke joinstyle="miter"></stroke><formulas><f eqn="if lineDrawn pixelLineWidth 0"></f><f eqn="sum @0 1 0"></f><f eqn="sum 0 0 @1"></f><f eqn="prod @2 1 2"></f><f eqn="prod @3 21600 pixelWidth"></f><f eqn="prod @3 21600 pixelHeight"></f><f eqn="sum @0 0 1"></f><f eqn="prod @6 1 2"></f><f eqn="prod @7 21600 pixelWidth"></f><f eqn="sum @8 21600 0"></f><f eqn="prod @7 21600 pixelHeight"></f><f eqn="sum @10 21600 0"></f></formulas><path oconnecttype="rect" gradientshapeok="t" oextrusionok="f"></path><lock aspectratio="t" vext="edit"></lock></shapetype><shape id="_x0000_i1025" type="#_x0000_t75" style="WIDTH: 120pt; HEIGHT: 201.75pt;"><imagedata otitle="" src="file:///C:\DOCUME~1\MACGEN~1\LOCALS~1\Temp\msohtml1\01\clip_image001.emz"></imagedata></shape></div></td></tr></tbody></table></textbox><wrap type="square"></wrap></shape>Procedure: Step 1. Production of high-titer virus in 293 cells 1.        Step 2. Infection of targeting cells edr Procedure illustration:

macgene 发表于 2009-1-5 09:56:00

迈晨科技（北京）有限公司竭诚为您服务！详情请登录网站：www.macgene.com，联系电话：010-82057786

macgene 发表于 2009-3-30 12:30:44

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详情请登录网站：www.macgene.com，联系电话：010-82057786

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[原创]逆转录病毒感染试剂盒