[原创]鼠尾DNA提取试剂盒
<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><a name="OLE_LINK30"></a><a name="OLE_LINK29"><span style="mso-bookmark: OLE_LINK30;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 16pt;"><font face="Times New Roman">Tail DNA Preparation</font></span></b></span></a><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 16pt;"><font face="Times New Roman"> Kit<span style="mso-spacerun: yes;"> </span></font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-SIZE: 16pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">鼠尾</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 16pt;"><font face="Times New Roman">DNA</font></span></b><b style="mso-bidi-font-weight: normal;"><span style="FONT-SIZE: 16pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">提取试剂盒</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 16pt;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 11pt;"><font face="Times New Roman">Catalogue</font></span><span style="FONT-SIZE: 11pt; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">：</span><font face="Times New Roman"><span lang="EN-US" style="FONT-SIZE: 11pt;">MNK001<span style="mso-spacerun: yes;"> </span>Storage: </span><span lang="IT" style="FONT-SIZE: 11pt; mso-ansi-language: IT;">4 ºC<p></p></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Kit Components:<b style="mso-bidi-font-weight: normal;"><p></p></b></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;"><p> </p></span></p><table class="MsoTableGrid" cellspacing="0" cellpadding="0" border="1" style="BORDER-RIGHT: medium none; BORDER-TOP: medium none; BORDER-LEFT: medium none; BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-yfti-tbllook: 480; mso-border-alt: solid windowtext .5pt; mso-border-insideh: .5pt solid windowtext; mso-border-insidev: .5pt solid windowtext;"><tbody><tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes;"><td valign="top" width="105" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 1pt solid; WIDTH: 78.75pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Component A<p></p></span></p></td><td valign="top" width="144" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 108pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Lysing Solution<p></p></span></p></td><td valign="top" width="60" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 45.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">50mL<p></p></span></p></td></tr><tr style="mso-yfti-irow: 1;"><td valign="top" width="105" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 1pt solid; WIDTH: 78.75pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Component B<p></p></span></p></td><td valign="top" width="144" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 108pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Salt Solution<p></p></span></p></td><td valign="top" width="60" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 45.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">25mL<p></p></span></p></td></tr><tr style="mso-yfti-irow: 2; mso-yfti-lastrow: yes;"><td valign="top" width="105" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 1pt solid; WIDTH: 78.75pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">MP023<p></p></span></p></td><td valign="top" width="144" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 108pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">Proteinase K <p></p></span></p></td><td valign="top" width="60" style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: #ece9d8; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: #ece9d8; WIDTH: 45.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; BACKGROUND-COLOR: transparent; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid windowtext .5pt;"><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;">1mL<p></p></span></p></td></tr></tbody></table><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;"><p> </p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; FONT-FAMILY: Verdana;"><p> </p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-fareast-font-family: Verdana; mso-bidi-font-family: Verdana;"><span style="mso-list: Ignore;">1.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Lyse cells 4 hours to overnight in 500ul Lysing Solution (with proteinase K and 2-</span><span lang="EN-US"><font face="Times New Roman"></font></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">mercaptoethanol). Incubate at 55 ºC.<p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 125%;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;"><p> </p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 36pt; LINE-HEIGHT: 125%;"><i style="mso-bidi-font-style: normal;"><span lang="EN-US" style="FONT-SIZE: 8pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Add fresh to aliquots of solution:<p></p></span></i></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 125%;"><i style="mso-bidi-font-style: normal;"><span lang="EN-US" style="FONT-SIZE: 8pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;"><span style="mso-tab-count: 1;"> </span>5ul 2-mercaptoethanol (not included) per 1ml lysing solution<p></p></span></i></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 125%;"><i style="mso-bidi-font-style: normal;"><span lang="EN-US" style="FONT-SIZE: 8pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;"><span style="mso-tab-count: 1;"> </span>20ul Proteinase K (20mg/ml) per 1ml lysing solution<p></p></span></i></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-fareast-font-family: Verdana; mso-bidi-font-family: Verdana;"><span style="mso-list: Ignore;">2.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Vortex 10 seconds. Add 250ul Salt Solution and vortex 5 seconds. Let stand 15 minutes on ice.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-fareast-font-family: Verdana; mso-bidi-font-family: Verdana;"><span style="mso-list: Ignore;">3.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Spin 15 min at maximum speed at 4 ºC<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-fareast-font-family: Verdana; mso-bidi-font-family: Verdana;"><span style="mso-list: Ignore;">4.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Transfer supernatant to clean tube and precipitate DNA with 750ul ethanol. Mix well. Pellet by spinning gently at room temperature at speed “<chmetcnv wst="on" unitname="”" sourcevalue="10" hasspace="False" negative="False" numbertype="1" tcsc="0">10”</chmetcnv> for 2-3 minutes.<p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; LINE-HEIGHT: 125%; TEXT-ALIGN: left; mso-pagination: widow-orphan; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-fareast-font-family: Verdana; mso-bidi-font-family: Verdana;"><span style="mso-list: Ignore;">5.<span style="FONT: 7pt "Times New Roman";"> </span></span></span><span lang="EN-US" style="FONT-SIZE: 9pt; LINE-HEIGHT: 125%; FONT-FAMILY: Verdana; mso-bidi-font-family: Arial;">Wash DNA pellet with 750ul 70% ethanol and air dry. Dissolve in 100ul TE or DNase-free water. Resuspend DNA rapidly by incubating 15 minutes at 55 ºC<p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> <p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">迈晨科技（北京）有限公司竭诚为您服务 ！<span lang="EN-US"><p></p></span></span></p><p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">详情请登录网站：<span lang="EN-US">www.macgene.com</span>，联系电话：<span lang="EN-US">010-82057786<p></p></span></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"></p></font></p></span></p><p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">迈晨科技（北京）有限公司竭诚为您服务 ！<span lang="EN-US"><p></p></span></span></p><p><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体;">详情请登录网站：<span lang="EN-US">www.macgene.com</span>，联系电话：<span lang="EN-US">010-82057786<p></p></span></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"></p> 顶一下，顶一下
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