macgene 发表于 2009-7-21 14:18:14

迈晨科技【供应】鼠尾DNA提取试剂盒

鼠尾DNA提取试剂盒
Catalogue：MNK001
Storage: 4 ordm;C
Kit Components:
Component A

Lysing Solution
50mL
Component B

Salt Solution
25mL

MP023

Proteinase K
1mL

1.
Lyse cells 4 hours to overnight in 500ul Lysing Solution (with proteinase K and 2- mercaptoethanol). Incubate at 55 ordm;C.
Add fresh to aliquots of solution:
5ul 2-mercaptoethanol (not included) per 1ml lysing solution
20ul Proteinase K (20mg/ml) per 1ml lysing solution
2.Vortex 10 seconds. Add 250ul Salt Solution and vortex 5 seconds. Let stand 15 minutes on ice.
3.Spin 15 min at maximum speed at 4 ordm;C
4.Transfer supernatant to clean tube and precipitate DNA with 750ul ethanol. Mix well. Pellet by spinning gently at room temperature at speed “10” for 2-3 minutes.
5.Wash DNA pellet with 750ul 70% ethanol and air dry. Dissolve in 100ul TE or DNase-free water. Resuspend DNA rapidly by incubating 15 minutes at 55 ordm;C
The assay has to be finished in 30 minutes after mixing the working solution and samples.
迈晨科技（北京）有限公司专业提供即用型液体装细胞培养基，产品全，分类细，小包装，适合各种细胞培养，此外供应支原体检测和清除试剂盒，标签抗体，缓冲液，抗生素，酶，蛋白质，免疫学试剂，并为Worthington酶和invivogen的北方代理商。产品原料进口，质量保证，价格适中，服务周到，欢迎广大朋友和客户网上订货www.macgene.com
订购电话：010-82057786

macgene 发表于 2009-7-27 14:55:16

好的，支持！

macgene 发表于 2009-8-3 13:02:17

不错，支持！

macgene 发表于 2009-8-17 10:19:25

:lol   顶！

macgene 发表于 2009-9-2 11:05:40

支持一下 ！

macgene 发表于 2009-9-22 15:39:13

:lol   顶 ！

macgene 发表于 2009-10-20 11:12:29

顶一下了~

macgene 发表于 2009-12-30 09:02:39

:lol顶！

macgene 发表于 2010-2-25 10:12:21

:victory:   支持一下~

macgene 发表于 2010-3-17 10:53:20

:victory: 不错
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