[注意]Cambrex蛋白分析

lzq888 发表于 2006-12-9 21:39:00

蛋白分析     Cambrex Bio Science Rockland, Inc Protein Analysis -----New! Versalinx?Protein Sample Preparation Tools 50602 Experimenter's Kit 50593 Versalinx Amine Modifying Reagent,10 mg 50594 Versalinx Carbohydrate Modifying Reagent,10 mg 50595 Versalinx Disulfide Forming Reagent,10 mg 50596 Versalinx Sulfhydral Alkylating Reagent,10mg 50592 Versalinx SHA-Agarose Chromotography Medium,5ml 描述： Versalinx Protein Sample Preparation Tools employ a unique small molecule, chemical affinity technology. The basis for this system is the specific interaction between two non-biological molecules, phenyl(di)boronic acid (P(D)BA) and salicylhydroxamic acid (SHA). Under conditions typical for bioseparation, Versalinx complex formation is robust and highly specific and enables the immobilization of P(D)BA conjugated biospecific ligands to SHA modified Agarose facilitating affinity separations. Features: >>>Easy-to-use ?Standard coupling reaction conditions. >>>Reversible ligand linkage under mild conditions ?Enables recovery of precious capture ligand or ligand-target complexes. >>>Small molecule based chemistry ?High retention of biological activity. Low non-specific binding, even for complex samples such as cell lysates. >>>Highly specific interaction between SHA and PDBA ?No need to purify sample. Effortless Implementation: >>>Quick and simple ?P(D)BA conjugate your capture ligand, bind ligand to column, capture and recover target protein. >>>P(D)BA modifi cation reagents ?Select site and degree of ligand modification. >>>SHA-modified Agarose Chromatography Media ?Universal: binds any PDBA-capture ligand. Now Available - Experimenter's Kit >>>Kit includes 5 mg of each Modifying Reagent >>>and 5 ml of SHA Chromotography Medium --New!!---Transport Protein Delivery Reagent 50568 Transport Protein Delivery Reagent,400 ug 描述： Transport Protein Delivery Reagent is a revolutionary new tool for non-cytotoxic delivery of biologically active cargo molecules (proteins, peptides, or small molecules) directly into live cells. Transport Reagent allows you to quickly and easily study cell and protein function, while avoiding the problems of DNA transfections (promoter interference, variable levels of transfection). This newly discovered peptide (patent pending) translocates itself as well as attached cargo molecules into cultured cells when added to the culture medium. Transport Reagent-Cargo Complex delivery is very efficient (up to 100% of cultured primary cells or cell lines) and allows rapid analysis of the unharmed transduced cells. Transport Reagent delivers: >>>Efficient Transfer ?Deliver biologically active proteins, peptides, and other macromolecules in up to 100% of cells. >>>Speed ?Deliver macromolecules into cells in less than 1-2 hours. >>>Flexibility ?Transfect a wide variety of cell types including primary cells. >>>Non-cytotoxicity - Assay live cells and ensure that the measured effect is due to your cargo, not due to the transfection method. >>>Ease of use ?Simply mix Transport Reagent with your protein and apply to cells. Several cell lines and primary cells have been successfully transfected with Transport Reagent, including: Human Bronchial Epithelial Cells >>>Human Epidermal Keratinocytes >>>Aortic Smooth Muscle Cells >>>HUVEC >>>Human Dermal Fibroblasts >>>NIH3T3 >>>293 >>>C2C12 The conjugation of Transport Reagent and a cargo molecule occurs through the 2-pyridyldithio group on the reagent which forms a disulfide bond with any free thiol (cysteine) situated on the cargo molecule. Coupling of Transport Reagent with cargo molecules is a simple process ?simply incubate appropriate amounts of each component at room temperature for 15-30 minutes, dilute with culture medium and apply to culturing cells. Transport Reagent is supplied as a lyophilized powder, which can be reconstituted with water, aliquoted and stored frozen. 400 ug of Transport Reagent is sufficient for 40-80 reactions in a 6-well or 35-mm dish, or 700-1,400 reactions in a 96-well plate. A Beta-Galactosidase control is supplied to allow testing and optimization of your experimental parameters. 励远科技有限公司(Cambrex部)联系人：李志强电话： 13959821799    QQ：648456366网址：www.leeyond.com     E-mail：     <a href="mailto:lzq598@yahoo.com.cn">lzq598@yahoo.com.cn</a>
[此贴子已经被作者于2007-5-7 21:43:49编辑过]

silent003 发表于 2014-12-18 09:21:45

英语不太好，分享的全英文不太好懂，但是说到蛋白分析我也分享一种仪器-特定蛋白分析仪。它从结构上主要分为透射比浊和散射比浊。
透射比浊，测定的是透射光和浊度的关系。结构简单，设计容易，但受入射光影响大，灵敏度低。
散射比浊，测定散射光和浊度的关系。灵敏度较高，但散射光因微粒大小和性质不同，可呈非均向散射，即不同的角度散射光的强度是不同的，因此利用散射比浊，不同的角度测定特性不同。大致说来，前散射因受血清中白蛋白，脂蛋白的影响小而常用，但不能排除乳糜的干扰。

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[注意]Cambrex蛋白分析