[公告]Grow´n´Glow ACE1 酵母双杂交系统
<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">特点和优点：</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">1.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">可以筛选不同蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">-</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">蛋白之间相互作用的强度；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">2.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">利用铜诱导的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">CUP1/ACE1</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">系统能更有效的检测体内蛋白相互作用；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">3.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">利用铜抗性这种严格的筛选，会出现很少的假阳性；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">4.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">利用</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">这种绿色荧光蛋白的报告基因，筛选阳性菌株可以节省大量的时间；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">5.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">筛选阳性菌株不需要外部酶底物，如</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">X-Gal</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;"><span style="mso-list: Ignore;"><font face="Times New Roman">6.<span style="FONT: 7pt 'Times New Roman';"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">适合高通量的筛选分析。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><b style="mso-bidi-font-weight: normal;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">产品介绍：</span></b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></b></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 21pt; mso-char-indent-count: 2.0;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">酵母双杂交系统是一种用于筛选体内蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">-</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">蛋白之间相互作用的方法，它依赖于</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">结合结构域和</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">激活结构域的分离，并将这两个结构域基因分别与两个蛋白基因融合在一起，如果两种蛋白能够相互作用，就会使这两个结构域重组成一个有活性的转录因子复合物，这样，就会使报告基因得以表达。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 21pt; mso-char-indent-count: 2.0;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">MoBiTec</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">ACE1</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">双杂交系统是一个强大的工具，它可以用于新基因的发现和功能分析，而且可以有效的鉴别体内蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">-</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">蛋白的作用。它将铜诱导的转录因子</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">ACE1</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">和铜抗性</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">CUP1</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">基因与作为报告基因绿色荧光蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">基因结合起来。两种相互作用的蛋白分别与</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">ACE1</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">结合结构域和</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">DNA</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">激活结构域相融合，从而使我们可以通过下面的方法筛选：</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">1.</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">在含铜的培养基上生长的细胞；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">2.</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">依赖铜表达的强荧光</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">可以通过紫外光检测到。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 21pt; mso-char-indent-count: 2.0;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-size: 10.5pt;">因此，这种新技术，可以很有效的通过一步法筛选可能的阳性表达，即在含有铜的培养基上生长并发出绿色荧光的菌株。它是一种快速、高灵敏度和更值得信赖的检测方法。不断改变铜浓度还可以筛选出不同蛋白之间相互作用的强度。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 21pt; mso-char-indent-count: 2.0;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p><font face="Times New Roman"> </font></p></span></p>
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