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求助:哪位高手知道大鼠的纹状体怎么取?

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7567686 发表于 2009-4-30 17:15:07 | 显示全部楼层 |阅读模式
小弟的实验需要取大鼠的纹状体,以前从来没操作过,有谁知道能指点一二,感激不尽!
glia 发表于 2009-5-1 01:10:06 | 显示全部楼层
看下大鼠脑图谱吧,你就会知道怎么做了。本站应该有图谱下载的,搜索下。不过,首先要有工具,像勺一样的,那个取起来就会方便很多。
 楼主| 7567686 发表于 2009-5-5 17:57:37 | 显示全部楼层
好的 ~~谢谢指点
thinker 发表于 2009-5-6 04:28:22 | 显示全部楼层
Brain striata preparation                a. Following behavioral testing, mice are sacrificed in a random order by cervical dislocation.
      b. Brains are quickly harvested and placed on ice.  
      c. The right and left striata are dissected (see Figures 1 A-I below), quickly frozen on dry ice, and then stored at -80°C freezer until analysis.
   

    Figure 1 A-I: Schematic dissection of the striatum for the determination of striatal neurotransmitters. Fig. 1-A: Dorso-ventral view of the harvested brain and the transection of the cerebellum. Fig. 1-B: The forebrain is transversely resected   at midline beginning at the red line. Fig. 1-C: Schematic cross-section of the brain showing the left and right regions of the striatum (green). Fig. 1-D: Using 2 stiff brushes, the partially halved brain in Fig. 1B is gently spread apart (yellow arrows). Fig. 1-E:While the left brush is used to stabilize the brain, the right brush iscarefully placed into the hippocampal groove (green arrow in Fig. 1D) and gently rotated until the hippocampus is displaced. Fig. 1-F: The hippocampus (hip) is shown displaced from its groove. Fig. 1-G:Using the brush to further undermine the brain, the right hippocampusis completely severed from the right cortex and in turn the cortex isflipped medial-laterally to expose the medial surface of the rightstriatum (demarcated by red lines). Fig. 1-H: Usingthe same brush, the lateral limit of the right striatum is bluntlydissected along its perimeter in order to move it medially forharvesting. Fig. 1-I: The right striatum is transected along the dashed red lines (Fig. 1H and I) using an iris scissor. Steps Figs. 1E-I are repeated for the left half of the brain to harvest the left striatum.
Brain_Striatum.jpg
 楼主| 7567686 发表于 2009-5-8 17:12:05 | 显示全部楼层
哇~~这个东西太好了~~我搞了本大鼠脑立体定位图谱好久都没搞懂啊~~
 楼主| 7567686 发表于 2009-5-8 17:15:20 | 显示全部楼层
谢谢~这个大哥了
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