Microcystin微囊藻毒素-LR单克隆抗体

Microcystin微囊藻毒素-LR单克隆抗体

微囊藻毒素Microcystin类产品09年末订购享受全国最优价!

Monoclonal Antibody to Microcystin-LR (MC10E7)

Product Specification

CLONE:    MC10E7  
ISOTYPE:    Mouse IgG1  
PURITY DETAIL:    Protein G-affinity purified.  
FORMULATION:    Lyophilized.  
RECONSTITUTION:    Reconstitute with 200μl PBS containing sodium azide.  
IMMUNOGEN:    Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.  
SPECIFICITY:    Recognizes all 4-Arg microcystins.  
APPLICATION:    ELISA (1:10'000-1:100'000)
Microcystin concentrations can be measured in the range of ~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1'000 times higher compared to UV detection.  
SHIPPING:    SHIPPED ON BLUE ICE  
LONG TERM STORAGE:    -20°C  
USE/STABILITY:    Storage at 4°C will decrease antibody activity and must be avoided.  
HANDLING:    Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.  

Product Description

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06μg/l have been obtained. The provisional guideline value proposed by the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.
The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1μg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.

Product Specific Literature References

Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001)
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001) Abstract

General Information

Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

    如有更多其它试剂产品需求与查询,请及时接洽：info@express-cn.com 或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/lyb.htm）留下您的查询内容，我们即会在当天为您作出回复。

伊普瑞斯科技有限公司
info@express-cn.com
在线客服：QQ：1069267096
          MSN:liyuqiu1983@hotmail.com
              cindylee-1981@hotmail.com
总机：010-60206266
传真：010-60206773
www.express-cn.com