PHI品牌的全称为PROHISTO,免疫组化及免疫荧光类产品,抗体扩增系列产品

PHI品牌的全称为PROHISTO,其生产基地位于南美哥伦比亚,主要生产免疫组化及免疫荧光类产品,现有抗体扩增系列产品如下:
      PHI-AA1-1        Antibody Amplifier™ 抗体扩增器（12槽，每槽一玻片）
      PHI-AA2-1        Antibody Amplifier Eclipse™抗体扩增器（非透光型）
      PHI-AA3-1        Amplifying Antibody Dilution Buffer™ 稀释液
      PHI-AA4-1        Amplifying IHC Wash Buffer™ (20X)  免疫组化冲洗液
      PHI-AA5-1        Epitope Unmasking Solution (10X) (500 ml) 抗原修复剂(恢复因预杂交或固定而导致抗原活性降低的抗原活性)
      PHI-AA6-1        Epitope Unmasking Solution (10X) (250 ml) 抗原修复剂
                                                                                      PHI-AAK-1-KI01        Antibody Amplifier™ Kit 1           抗体扩增试剂盒1
- 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
PHI-AAK-2-KI01        Antibody Amplifier Eclipse™ Kit 2    抗体扩增试剂盒2（适用于IF和活细胞实验）
    - 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
PHI-AAK-3-KI01        Antibody Amplifier™ / Antibody Amplifier Eclipse™ Kit 3 抗体扩增器/抗体扩增试剂盒3
    - 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
PHI-AAK-4-KI01        Super Amplifying IHC Kit with Antibody Amplifier™高级抗体扩增免疫组化试剂盒
    - 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (250 ml) (Prod. No. PHI-AA6)
即PHI-AAK-KI01 + PHI-AA6-1
PHI-AAK-5-KI01        Super Amplifying IF Kit with Antibody Amplifier Eclipse™高级抗体扩增免疫荧光试剂盒
- 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (250 ml) (Prod. No. PHI-AA6)
即PHI-AAK-2-KI01 + PHI-AA6-1
PHI-AAK-6-KI01        Super Amplifying IHC/IF Kit with Antibody Amplifier™ and Antibody Amplifier Eclipse™
高级抗体扩增两用试剂盒
    - 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (250 ml) (Prod. No. PHI-AA6)
即PHI-AAK-3-KI01 + PHI-AA6
PHI-AAK-7-KI01        Ultra Amplifying IHC Kit (AA1) for Cell Stress (150 slides)
用于细胞应激反应的抗体超级扩增免疫组化试剂盒（AAK-4加4种单抗）
  - 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (250 ml) (Prod. No. PHI-AA6)
- Monoclonal Antibody to p53
- Monoclonal Antibody to p53 (pSer15)
- Monoclonal Antibody to DNA/RNA Damage   DNA/RNA损伤单抗
- Monoclonal Antibody to Nitrotyrosine         硝化酪氮酸单抗
PHI-AAK-8-KI01        Ultra Amplifying IF Kit (AA2) for Cell Stress (150 slides)
用于细胞应激反应的抗体超级扩增免疫荧光试剂盒（AAK-5加4种单抗）
  - 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (250 ml) (Prod. No. PHI-AA6)
- Monoclonal Antibody to p53
- Monoclonal Antibody to p53 (pSer15)
- Monoclonal Antibody to DNA/RNA Damage
- Monoclonal Antibody to Nitrotyrosine
PHI-AAK-9-KI01        Ultra Amplifying IHC Kit (AA1) for Cell Stress (300 slides)
用于细胞应激反应的抗体超级扩增免疫组化试剂盒
- 1 Antibody Amplifier™ (Prod. No. PHI-AA1)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (500 ml) (Prod. No. PHI-AA5)
- Monoclonal Antibody to p53
- Monoclonal Antibody to p53 (pSer15)
- Monoclonal Antibody to DNA/RNA Damage
- Monoclonal Antibody to Nitrotyrosine
PHI-AAK-10-KI01        Ultra Amplifying IF Kit (AA2) for Cell Stress (300 slides)
用于细胞应激瓜的抗体超级扩增免疫荧光试剂盒
- 1 Antibody Amplifier Eclipse™ (Prod. No. PHI-AA2)
- 1 Bottle of Amplifying Antibody Dilution Buffer™ (Prod. No. PHI-AA3)
- 1 Bottle of Amplifying IHC Wash Buffer™ (20X) (Prod. No. PHI-AA4)
- 1 Bottle of Epitope Unmasking Solution (10X) (500 ml) (Prod. No. PHI-AA5)
- Monoclonal Antibody to p53
- Monoclonal Antibody to p53 (pSer15)
- Monoclonal Antibody to DNA/RNA Damage
- Monoclonal Antibody to Nitrotyrosine
产品特点：节约时间、节省成本、可重复性强、灵敏性高、价格便宜；
         细胞实验室、免疫学实验室、动物实验室

试剂盒的操作步骤：
1. Deparaffinize slides (optional to use the Antibody AmplifierTM).       脱蜡
2. Wash (optional to use the Antibody AmplifierTM)
       冲洗（加含福尔马林类冲洗液AA4固定细胞，其中可能抗原的活性会被屏蔽）
3. Antigen retrieval                                     抗原修复（加抗原修复剂AA5/6恢复抗原的活性）
4. Block (optional to use the Antibody AmplifierTM)          阻断也称预杂交（背景清零）
5. Primary antibody (use the Antibody AmplifierTM)          添加一抗
6. Place the Antibody AmplifierTM on a standard rocker, place the lid on the chamber,and incubate while rocking.
将抗体扩增器放在摇床上，盖上盖子，边摇动边孵育。
7. Wash (optional to use the Antibody AmplifierTM).           冲洗
8. Secondary antibody (use the Antibody AmplifierTM)         添加二抗
9. Wash (optional to use the Antibody AmplifierTM).           冲洗
10. Substrate                                           添加显色剂
11. Counter-stain                                        复染


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