[转贴]PKA regulates calcium permeability.......

①标题：Protein kinase A regulates calcium permeability of NMDA receptorsfficeffice" />

②作者：V Arvydas Skeberdis^1, 3, 4, Vivien Chevaleyre^1, 4, C Geoffrey Lau^1, 4, Jesse H Goldberg^2, 3, Diana L Pettit¹, Sylvia O Suadicani¹, Ying Lin¹, Michael V L Bennett¹, Rafael Yuste², Pablo E Castillo¹ & R Suzanne Zukin¹

③来源：<A href="javascript:AL_get(this,%20'jour',%20'Nat%20Neurosci.');">Nat Neurosci. 2006 Mar 12

④发表/制作时间：

⑤内容简介：Calcium (Ca²⁺) influx through NMDA receptors (NMDARs) is essential for synaptogenesis, experience-dependent synaptic remodeling and plasticity. The NMDAR-mediated rise in postsynaptic Ca²⁺ activates a network of kinases and phosphatases that promote persistent changes in synaptic strength, such as long-term potentiation (LTP). Here we show that the Ca²⁺ permeability of neuronal NMDARs is under the control of the cyclic AMP–protein kinase A (cAMP-PKA) signaling cascade. PKA blockers reduced the relative fractional Ca²⁺ influx through NMDARs as determined by reversal potential shift analysis and by a combination of electrical recording and Ca²⁺ influx measurements in rat hippocampal neurons in culture and hippocampal slices from mice. In slices, PKA blockers markedly inhibited NMDAR-mediated Ca²⁺ rises in activated dendritic spines, with no significant effect on synaptic current. Consistent with this, PKA blockers depressed the early phase of NMDAR-dependent LTP at hippocampal Schaffer collateral–CA1 (Sch-CA1) synapses. Our data link PKA-dependent synaptic plasticity to Ca²⁺ signaling in spines and thus provide a new mechanism whereby PKA regulates the induction of LTP.