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Monoclonal Antibody to Microcystin-LR (MC10E7)
规格：200 μg
Product Specifications CLONE: MC10E7 ISOTYPE: Mouse IgG1 IMMUNOGEN: Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin. SPECIFICITY: Recognizes all 4-Arg microcystins. APPLICATION: ELISA (1:10'000-1:100'000) Microcystin concentrations can be measured in the range of ~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1'000 times higher compared to UV detection. FORMULATION: Lyophilized from antibody purified by protein G affinity chromatography. RECONSTITUTION: Reconstitute with 200μl PBS containing sodium azide. SHIPPING: SHIPPED ON BLUE ICE LONG TERM STORAGE: -20°C USE/STABILITY: Storage at 4°C will decrease antibody activity and must be avoided. HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. Product Description With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06 μg/l have been obtained. The provisional guideline value proposed by the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7 x 1010 l/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1 μg/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water. 伊普瑞斯科技有限公司 联系人:刘小姐 info@express-cn.com 010-60206266 www.express-cn.com | 
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