免疫荧光图

Microtubules (green) and actin microfilaments (red) were fluoresently stained and imaged with a confocal microscope. Guidance cues control growth cone motility and axon guidance by regulating these two key cytoskeletal elements. Image courtesy of Paul Letourneau, University of MN.

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Phase contrast microscopy allows filopodia of less than 500 nm in diameter to be observed in a non-destructive manner. Using this technique, filopodia of greater then 100 microns in length have been observed to stimulate complex changes in growth cone behavior (see Gomez and Letourneau, J Neurosci 14, 5959-5972). Scale bar,10 µm

 

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More on the website:



http://gomez.anatomy.wisc.edu/