日志
各种激发光和吸收光波长
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现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。
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Excitation and Emission Wavelengths of Fluorophores
Tavi\'s \"FluoroTable\": Common fluorophores
(from Zeiss Corporation website)
|
Fluorophore |
Absorption |
Emission |
Other info |
|
1,5 IAEDANS |
336 |
490 |
|
| 1,8-ANS |
372 |
480 |
|
| 4-Methylumbelliferone |
385
|
502 |
|
| 5-carboxy-2,7-dichlorofluorescein |
504 |
529 |
|
| 5-Carboxyfluorescein (5-FAM) |
492 |
518 |
|
| 5-Carboxynapthofluorescein (pH 10) |
512/598 |
563/668 |
Ratio Dye, pH |
| 5-Carboxytetramethylrhodamine (5-TAMRA) |
542 |
568 |
|
| 5-FAM (5-Carboxyfluorescein) |
492 |
518 |
|
| 5-HAT (Hydroxy Tryptamine) |
370-415 |
520-540 |
|
| 5-Hydroxy Tryptamine (HAT) |
370-415 |
520-540 |
|
| 5-ROX (carboxy-X-rhodamine) |
578 567 |
604 591 |
|
| 5-TAMRA (5-Carboxytetramethylrhodamine) |
548 542 |
552 568 |
|
| 6-Carboxyrhodamine 6G |
518 |
543 |
|
| 6-CR 6G |
518 |
543 |
|
| 6-JOE |
520 |
548 |
|
| 7-Amino-4-methylcoumarin |
351 |
430 |
|
| 7-Aminoactinomycin D (7-AAD) |
546 |
647 |
|
| 7-Hydroxy-4-methylcoumarin |
360 |
449, 455 |
|
| 9-Amino-6-chloro-2-methoxyacridine |
412, 430 |
471, 474 |
|
| ABQ |
344 |
445 |
|
| Acid Fuchsin |
540 |
630 |
|
| ACMA (9-Amino-6-chloro-2-methoxyacridine) |
412, 430 |
471, 474 |
|
| Acridine Orange + DNA |
502 |
526 |
|
| Acridine Orange + RNA |
460 |
650 |
|
| Acridine Orange, both DNA & RNA |
440-480 |
520-650 |
|
| Acridine Red |
455-600 |
560-680 |
|
| Acridine Yellow |
470 |
550 |
|
| Acriflavin |
436 |
520 |
|
| Acriflavin Feulgen SITSA |
355-425 |
460 |
|
| Aequorin (Photoprotein) |
|
466 |
Photoprotein |
| AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP |
|
||
| Alexa Fluor 350 |
346 342 |
442 441 |
|
| Alexa Fluor 430 |
431 |
540 |
|
| Alexa Fluor 488 |
495, 492 |
519, 520 |
|
| Alexa Fluor 532 |
531, 532 |
553, 554 |
|
| Alexa Fluor 546 |
556, 557 |
572, 573 |
|
| Alexa Fluor 568 |
577, 578 |
603 |
|
| Alexa Fluor 594 |
590, 594 |
617, 618 |
|
| Alexa Fluor 633 |
632 |
650 |
|
| Alexa Fluor 647 |
647 |
666 |
|
| Alexa Fluor 660 |
668 |
698 |
|
| Alexa Fluor 680 |
679 |
702 |
|
| Alizarin Complexon |
530-560, 580 |
580, 624-645 |
|
| Alizarin Red |
530-560 |
580 |
|
| Allophycocyanin (APC) |
630, 645 |
655, 660 |
|
| AMC, AMCA-S |
345 |
445 |
|
| AMCA (Aminomethylcoumarin) |
345 347 |
425 444 |
|
| AMCA-X |
353 |
442 |
|
| Aminoactinomycin D |
555 |
655 |
|
| Aminocoumarin |
346 350 |
442 445 |
|
| Aminomethylcoumarin (AMCA) |
345 347 |
425 444 |
|
| Anilin Blue |
|
600 |
|
| Anthrocyl stearate |
360-381 |
446 |
|
| APC (Allophycocyanin) |
630, 645 |
655, 660 |
|
| APC-Cy7 |
625-650 |
755 |
|
| APTRA-BTC = Ratio Dye, Zn2+ |
466/380 |
520/530 |
Ratio Dye, Zn2+ |
| APTS |
424 |
505 |
|
| Astrazon Brilliant Red 4G |
500 |
585 |
|
| Astrazon Orange R |
470 |
540 |
|
| Astrazon Red 6B |
520 |
595 |
|
| Astrazon Yellow 7 GLL |
450 |
480 |
|
| Atabrine |
436 |
490 |
|
| ATTO-TAG? CBQCA |
465 |
560 |
|
| ATTO-TAG? FQ |
486 |
591 |
|
| Auramine |
460 |
550 |
|
| Aurophosphine G |
450 |
580 |
|
| Aurophosphine |
450-490 |
515 |
|
| BAO 9(Bisaminophenyloxadiazole) |
365 |
395 |
|
| BCECF (high pH) |
492, 503 |
520,528 |
|
| BCECF (low pH) |
482 |
520 |
|
| Berberine Sulphate |
430 |
550 |
|
| Beta Lactamase |
409 |
447, 520 |
|
| BFP blue shifted GFP (Y66H) Blue Fluorescent Protein |
381, 382, 383 |
445, 447, 448 |
blue shifted GFP (Y66H) Blue Fluorescent Protein |
| BFP/GFP FRET |
|
|
|
| Bimane |
398 |
490 |
|
| Bisbenzamide |
360 |
461 |
|
| Bisbenzimide (Hoechst) |
360 |
461 |
|
| bis-BTC = Ratio Dye, Zn2+ |
455/405 |
529/505 |
Ratio Dye, Zn2+ |
| Blancophor FFG |
390 |
470 |
|
| Blancophor SV |
370 |
435 |
|
| BOBO?-1 |
462 |
481 |
|
| BOBO?-3 |
570 |
602 |
|
| Bodipy 492/515 |
490 |
515 |
|
| Bodipy 493/503 |
533 |
549 |
|
| Bodipy 500/510 |
509 |
515 |
|
| Bodipy 505/515 |
502 |
510 |
|
| Bodipy 530/550 |
528 |
547 |
|
| Bodipy 542/563 |
543 |
563 |
|
| Bodipy 558/568 |
558 |
569 |
|
| Bodipy 564/570 |
564 |
570 |
|
| Bodipy 576/589 |
579 |
590 |
|
| Bodipy 581/591 |
584 |
592 |
|
| Bodipy 630/650-X |
625 |
642 |
|
| Bodipy 650/665-X |
647 |
665 |
|
| Bodipy 665/676 |
605 |
676 |
|
| Bodipy Fl |
504, 505 |
511, 513 |
|
| Bodipy FL ATP |
505 |
514 |
|
| Bodipy Fl-Ceramide |
505 |
511 |
|
| Bodipy R6G SE |
528 |
547 |
|
| Bodipy TMR |
542 |
574 |
|
| Bodipy TMR-X conjugate |
544 |
573 |
|
| Bodipy TMR-X, SE |
544 |
570 |
|
| Bodipy TR |
589 |
617 |
|
| Bodipy TR ATP |
591 |
620 |
|
| Bodipy TR-X SE |
588 |
616 |
|
| BO-PRO?-1 |
462 |
481 |
|
| BO-PRO?-3 |
544 |
570 |
|
| Brilliant Sulphoflavin FF |
430 |
520 |
|
| BTC - Ratio Dye Ca2+ |
464/401 |
533/529 |
Ratio Dye Ca2+ |
| BTC-5N - atio Dye, Zn2+ |
459/417 |
517/532 |
Ratio Dye, Zn2+ |
| Calcein |
494 |
517 |
|
| Calcein Blue |
373 |
440 |
|
| Calcium Crimson |
588, 589 |
611, 615 |
|
| Calcium Green |
501, 506 |
531 |
|
| Calcium Green-1 Ca2+ Dye |
506 |
531 |
Ca2+ Dye |
| Calcium Green-2 Ca2+ |
506/503 |
536 |
Ca2+ |
| Calcium Green-5N Ca2+ |
506 |
532 |
Ca2+ |
| Calcium Green-C18 Ca2+ |
509 |
530 |
Ca2+ |
| Calcium Orange |
549 |
575 576 |
|
| Calcofluor White |
385, 395, 405 |
437, 440, 445 |
|
| Carboxy-X-rhodamine (5-ROX) |
576 |
601 |
|
| Cascade Blue |
377 398 399 |
420 423 |
|
| Cascade Yellow |
399 400 |
550 552 |
|
| Catecholamine |
410 |
470 |
|
| CCF2 (GeneBlazer) |
|
|
|
| CFDA |
494 |
520 |
|
| CFP - Cyan Fluorescent Protein |
430, 433, 436, (453) |
474, 475, 476, (501) |
Cyan Fluorescent Protein |
| CFP/YFP FRET |
|
|
|
| Chlorophyll |
480 |
650 |
|
| Chromomycin A |
436-460 |
470 |
|
| Chromomycin A |
445 |
575 |
|
| CL-NERF (Ratio Dye, pH) |
504/514 |
540 |
Ratio Dye, pH |
| CMFDA |
494 |
520 |
|
| Coelenterazine Ca2+ Dye, bioluminescence |
(429) |
465 |
Ca2+ Dye, bioluminescence, native molecule |
| Coelenterazine cp (Ca2+ Dye,) |
(430) |
442 |
Ca2+ Dye, bioluminescence |
| Coelenterazine f |
(437) |
473 |
Ca2+ Dye, bioluminescence |
| Coelenterazine fcp |
|
452 |
Ca2+ Dye, bioluminescence |
| Coelenterazine h |
(437) |
464 |
Ca2+ Dye, bioluminescence |
| Coelenterazine hcp |
(433) |
444 |
Ca2+ Dye, bioluminescence |
| Coelenterazine ip |
|
441 |
Ca2+ Dye, bioluminescence |
| Coelenterazine n |
(431) |
467 |
Ca2+ Dye, bioluminescence |
| Coelenterazine O |
460 |
575 |
|
| Coumarin Phalloidin |
387 |
470 |
|
| C-phycocyanine |
|
|
|
| CPM Methylcoumarin |
384 |
469 |
Methylcoumarin |
| CTC |
400-450 |
602 |
|
| CTC Formazan |
|
|
|
| Cy2 |
489 |
506 |
|
| Cy3.1 8 |
554 |
568 |
|
| Cy3.5 |
581 |
598 |
|
| Cy3 |
514 552 554 |
566 570 |
|
| Cy5.1 8 |
649 |
666 |
|
| Cy5.5 |
675 |
695 |
|
| Cy5 |
649 |
666 670 |
|
| Cy7 |
710, 743 |
767, 805 |
|
| Cyan GFP |
433 (453) |
475 (501) |
|
| cyclic AMP Fluorosensor (FiCRhR) |
500 |
517 |
|
| CyQuant Cell Proliferation Assay |
480 |
520 |
Cell Proliferation Assay |
| Dabcyl |
453 |
|
|
| Dansyl |
340 |
578 |
|
| Dansyl Amine |
337 |
517 |
|
| Dansyl Cadaverine |
335 |
518 |
|
| Dansyl Chloride |
372 |
518 |
|
| Dansyl DHPE |
336 |
517 |
|
| Dansyl fluoride |
356 |
none |
|
| DAPI |
359 |
461 |
|
| Dapoxyl |
403 |
580 |
|
| Dapoxyl 2 |
374 |
574 |
|
| Dapoxyl 3 |
373 |
574 |
|
| DCFDA |
504 |
529 |
|
| DCFH (Dichlorodihydrofluorescein Diacetate) |
505 |
535 |
|
| DDAO |
463 |
607 |
|
| DHR (Dihydorhodamine 123) |
505 |
534 |
|
| Di-4-ANEPPS |
496 |
705 |
|
| Di-8-ANEPPS (non-ratio) |
488 498 |
605 713 |
|
| DiA (4-Di-16-ASP) |
456 |
591 |
|
| Dichlorodihydrofluorescein Diacetate (DCFH) |
505 |
535 |
|
| DiD - Lipophilic Tracer |
644 |
665 |
Lipophilic Tracer |
| DiD (DiIC18(5)) |
644 |
665 |
|
| DIDS |
341 |
415 |
|
| Dihydorhodamine 123 (DHR) |
505 |
535 |
|
| DiI (DiIC18(3)) |
549, 551 |
565 |
|
| Dinitrophenol |
349 |
|
|
| DiO (DiOC18(3)) |
484, 487 |
501, 502 |
|
| DiR |
748 |
780 |
Lipophilic Tracer |
| DiR (DiIC18(7)) |
750 |
779 |
|
| DM-NERF (high pH) |
497/510 |
540 |
Ratio Dye, pH |
| DNP |
349 |
|
|
| Dopamine |
340 |
490-520 |
|
| DsRed |
558 |
583 |
Red fluorescent protein |
| DTAF |
494 |
520 |
|
| DY-630-NHS |
621 |
660 |
Hemicyane label for proteins and DNA |
| DY-635-NHS |
634 |
664 |
Hemicyane label for proteins and DNA |
| EBFP |
383 |
447 |
Enhanced Blue Fluorescent Protein |
| ECFP |
436 |
474 |
Enhanced Cyan Fluorescent Protein |
| EGFP |
488, 498 |
507, 516 |
Enhanced Green Fluorescent Protein |
| ELF 97 |
345 |
530 |
|
| Eosin |
524 |
545 |
|
| Erythrosin |
529, 532 |
554, 555 |
|
| Erythrosin ITC |
529 |
555 |
|
| Ethidium Bromide |
510, 523 |
595, 605 |
|
| Ethidium homodimer -1 (EthD-1) |
528 |
617 |
|
| Euchrysin |
430 |
540 |
|
| EukoLight |
|
|
|
| Europium (III) chloride |
|
|
|
| EYFP |
513, 520 |
527, 532 |
Enhanced Yellow Fluorescent Protein |
| Fast Blue |
360 |
440 |
|
| FDA |
494 |
520 |
|
| Feulgen (Pararosaniline) |
570 |
625 |
|
| FIF (Formaldehyd Induced Fluorescence) |
405 |
433 |
|
| FITC |
490, 494 |
520, 525 |
|
| FITC Antibody |
493 |
517 |
|
| Flazo Orange |
375-530 |
612 |
|
| Fluo-3 |
480 ?506, 506 |
520, 527 |
|
| Fluo-4 |
494 |
516 |
|
| Fluorescein (FITC) |
490, 494 |
520, 525 |
|
| Fluorescein Diacetate |
494 |
520 |
|
| Fluoro-Emerald |
495 |
524 |
|
| Fluoro-Gold (Hydroxystilbamidine) |
361 |
536 |
|
| Fluor-Ruby |
555 |
582 |
|
| FluorX |
494 |
520 |
|
| FM 1-43 |
479 |
598 |
|
| FM 4-46 |
515 |
640 |
|
| Fura Red?(high pH) |
572 |
657 |
|
| Fura Red?Fluo-3 |
|
|
|
| Fura-2, high calcium |
335 |
505 |
Excitation ratio dye |
| Fura-2, low calcium |
363 |
512 |
Excitation ratio dye |
| Fura-2/BCECF |
|
|
|
| Genacryl Brilliant Red B |
520 |
590 |
|
| Genacryl Brilliant Yellow 10GF |
430 |
485 |
|
| Genacryl Pink 3G |
470 |
583 |
|
| Genacryl Yellow 5GF |
430 |
475 |
|
| GeneBlazer (CCF2) |
|
|
|
| GFP (S65T) |
498 |
516 |
|
| GFP red shifted (rsGFP) |
498 |
516 |
|
| GFP wild type, non-UV excitation (wtGFP) |
475 |
509 |
|
| GFP wild type, UV excitation (wtGFP) |
395 |
509 |
|
| GFPuv |
385 |
508 |
|
| Gloxalic Acid |
405 |
460 |
|
| Granular Blue |
355 |
425 |
|
| Haematoporphyrin |
530-560 |
580 |
| Hoechst 33258 |
345 |
487 |
|
| Hoechst 33342 |
347 |
|
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Filter sets installed on the Axiovert 200M: 1) Brightfield DIC: filter cube contains DIC analyzer 2) DAPI / BFP: Chroma set 31041, Ex at 380 nm (bandwidth 30 nm), Em at 460 nm (bandwidth 50 nm). 3) FITC / GFP: Chroma set 41001, Ex at 480 nm (bandwidth 40 nm), Em at 535 nm (band width 50 nm). 4) RHOD: Chroma set 41002c, Ex at 545 nm (bandwidth 30 nm), Em at 620 nm (bandwidth 60 nm). 5) Cy5: Chroma set 41024, Ex at 620 nm (bandwidth 60 nm), Em 665 nm and above (long pass filter). Also available upon request: 4) GFP: Chroma set 41017, Ex at 470 nm (bandwidth 40 nm), Em 525 nm (band width 50 nm). 5) CFP: Chroma set 31044v2, Ex at 436 nm (bandwidth 20 nm), Em 480 nm (bandwidth 40 nm). 6) YFP: Chroma set 41028, Ex at 500 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).
7) CFP/YFP FRET: Chroma set 31052, Ex 436 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).
