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<p>Vitellogenin (Carp) ELISA Kit<br/>Wako<br/> 300-08751Grade: <br/>Regulation / Hazardous: <br/>Storage condition: Keep at 2-10 degrees C.<br/>Manufacturer: Trans Genic Inc. <br/>Summary<br/> This product is for research use only. Do not administer it to human.<br/>Vitellogenin (Vg) is a precursor of egg york and detected female<br/>serum near ovulation. Vg is not detected in male serum, but treated<br/>with 17?-estradiol, Vg is detectable in male<br/>serum. </p><p>Manufactured by Trans Genic Inc. (Kumamoto, Japan)</p><p>Carp Vg ELISA kit is constructed as a sandwich ELISA format<br/>using anti carp Vg antibodies and titrate serum carp Vg.<br/>Kit Contents:</p><p>(1) Antibody-coated microtiter plate (8 wells x 12): 1<br/>plate</p><p>(2) The standard, Carp Vg, lyophilized: 2 vials</p><p>(3) Standard diluent: 30 mL</p><p>(4) HRP-conjugated anti Carp Vg antibody (x 500): 25 ?L</p><p>(5) Substrate buffer: 15 mL</p><p>(6) OPD (o-Phenylenediamine) tablets: 2 tablets</p><p>(7) Wash solution (x 20): 30 mL</p><p>(8) Stop solution: 6 mL<br/>Required Apparatuses:</p><p>(1) A microplate reader</p><p>(2) A micropipet</p><p>(3) A microplate washer<br/>Principle:</p><p>This kit is constructed using the antibodies specific to carp<br/>Vg, as both capture and secondary antibodies of sandwich ELISA format. <br/>During the assay, carp Vg in the samples is captured by the antibody, immobilized<br/>on the surface of microplate wells, and simultaneously reacts with the<br/>horseradish peroxidase (HRP) labeled antibody. Quantitation of carp<br/>Vg in samples can be made by measurement of HRP activity of the complex,<br/>which is exponential to the quantity of carp Vg.<br/>Features:</p><p>(1) Sensitivity</p><p>Dynamic range: 7.8 - 500<br/>ng/mL</p><p>(2) Recovery of exogenous vitellogenin</p><p>Plasma: Av. 95.6%<br/>Expired Date: 1 month after manufacturing</p><p>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</p><p> 如有更多其它试剂产品需求与查询,请及时接洽：<a href="mailto:info@express-cn.com">info@express-cn.com</a> 或在我公司查询主页客户留言处（<a href="http://www.express-cn.com/expressgb/lyb.htm">http://www.express-cn.com/expressgb/lyb.htm</a>）留下您的查询内容，我们即会在当天为您作出回复。</p><p>伊普瑞斯科技有限公司<br/><a href="mailto:info@express-cn.com">info@express-cn.com</a><br/>总机：010-60206266<br/>传真：010-60206773<br/><a href="http://www.express-cn.com">www.express-cn.com</a><br/></p>
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