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Vitellogenin (Carp) ELISA Kit
Wako
 300-08751Grade: 
Regulation / Hazardous: 
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Trans Genic Inc.
Summary
 This product is for research use only. Do not administer it to human.
Vitellogenin (Vg) is a precursor of egg york and detected female
serum near ovulation. Vg is not detected in male serum, but treated
with 17?-estradiol, Vg is detectable in male
serum.

Manufactured by Trans Genic Inc. (Kumamoto, Japan)

Carp Vg ELISA kit is constructed as a sandwich ELISA format
using anti carp Vg antibodies and titrate serum carp Vg.
Kit Contents:

(1) Antibody-coated microtiter plate (8 wells x 12): 1
plate

(2) The standard, Carp Vg, lyophilized: 2 vials

(3) Standard diluent: 30 mL

(4) HRP-conjugated anti Carp Vg antibody (x 500): 25 ?L

(5) Substrate buffer: 15 mL

(6) OPD (o-Phenylenediamine) tablets: 2 tablets

(7) Wash solution (x 20): 30 mL

(8) Stop solution: 6 mL
Required Apparatuses:

(1) A microplate reader

(2) A micropipet

(3) A microplate washer
Principle:

This kit is constructed using the antibodies specific to carp
Vg, as both capture and secondary antibodies of sandwich ELISA format.
During the assay, carp Vg in the samples is captured by the antibody, immobilized
on the surface of microplate wells, and simultaneously reacts with the
horseradish peroxidase (HRP) labeled antibody. Quantitation of carp
Vg in samples can be made by measurement of HRP activity of the complex,
which is exponential to the quantity of carp Vg.
Features:

(1) Sensitivity

Dynamic range: 7.8 - 500
ng/mL

(2) Recovery of exogenous vitellogenin

Plasma: Av. 95.6%
Expired Date: 1 month after manufacturing

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