[推荐]酵母双杂交系统
<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">特点与优点：</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">1.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">鉴定体内蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">-</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">蛋白相互作用：确定一个挑选的引诱蛋白与一个由</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">cDNA</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">文库表达的蛋白结合区域；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">2.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">阳性菌落发出绿色荧光，通过一步就可筛选出可能的阳性；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">3.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">不需要烦琐的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">β-</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">半乳糖苷酶检测；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">4.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">通过文库半乳糖诱导性表达筛选存在潜在毒性的蛋白相互作用；</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt 18pt; TEXT-INDENT: -18pt; mso-list: l0 level1 lfo1; tab-stops: list 18.0pt;"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt; mso-fareast-font-family: "Times New Roman";"><span style="mso-list: Ignore;"><font face="Times New Roman">5.<span style="FONT: 7pt "Times New Roman";"> </span></font></span></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">很少的假阳性。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">产品介绍：</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">这个双杂交系统提供了一个高技术和巧妙的方法来研究蛋白相互作用，另外，能表现出蛋白相互作用时的结构域、氨基酸残基和必须的条件，最终可确定新的蛋白，这种筛选能力使其成为基因发现和功能分析的强大工具。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">这个系统简化和加快了筛选过程，它将报告蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">和</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">LexA</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">蛋白结合起来构建了酵母双杂交系统。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">蛋白的相互作用，导致了两个报告基因（</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">LEU2</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">营养筛选基因和</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">基因）的表达，</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">LEU2</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">的表达，使酵母可以在缺少亮氨酸的培养基上生长，而</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">基因的表达，则可使酵母在紫外光下发出绿色荧光。</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 15.75pt; mso-char-indent-count: 1.5;"><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">使用绿色荧光蛋白</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">作为报告元件避免了费时的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">β-</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">半乳糖苷酶检测，特殊的</span><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><font face="Times New Roman">GFP</font></span><span style="FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">变异体由</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">pGNG1</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">质粒表达的，它比野生型</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">GFP</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">发出的荧光要强</span><span lang="EN-US" style="COLOR: black;"><font face="Times New Roman">18</font></span><span style="COLOR: black; FONT-FAMILY: 宋体; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman";">倍，两种蛋白相互作用的酵母菌落发出的绿色荧光可以很容易的被检测出来。</span><span lang="EN-US" style="COLOR: black;"><p></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><font face="Times New Roman"><span lang="EN-US" style="mso-bidi-font-size: 10.5pt;"><span style="mso-spacerun: yes;"> </span></span><span lang="EN-US">PSKAN<span style="mso-spacerun: yes;"> </span>pSKAN Phagemid Display System</span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><span style="mso-spacerun: yes;"><font face="Times New Roman"></font></span></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PS1255<span style="mso-spacerun: yes;"> </span>18mer-sequencing primer 1255 500 pmole</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> </font></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PS2897<span style="mso-spacerun: yes;"> </span>19mer-sequencing primer 2897 500 pmole</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> </font></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PSKAN3<span style="mso-spacerun: yes;"> </span>pIII antibody (monoclonal mouse IgG1) 100 μl</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> </font></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PSKAN2<span style="mso-spacerun: yes;"> </span>anti-hPSTI antibody (monoclonal mouse IgG1) 100 μl</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> </font></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PSKAN8<span style="mso-spacerun: yes;"> </span>pSKAN8 vector DNA, lyophilized 5 μg</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman"> </font></p></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><font face="Times New Roman">PSKAN4<span style="mso-spacerun: yes;"> </span>pMAMPF3-PSTI4 vector DNA, lyophilized 5 μg</font></span></p>
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