酪胺酸酶Tyrosinase from mushroom

酪胺酸酶Tyrosinase from mushroom

BioChemika, lyophilized, ≥2000 units/mg

Descriptions

Other Notes  Kinetic study of the suicide inactivation of tyrosinase1 ; Chromogenic assay

Unit Definition  1 U corresponds to the amount of enzyme which increases the absorbance at 305 nm by 0.001 per minute at pH 6.5 and 25°C (L-tyrosine as substrate, 3.0 mL reaction mix); 30 absorbance-U as described above are equivalent to ~1 U [when 1 U is the amount of enzyme which oxidyzes 1 μmol 4-methylcatechol per minute at pH 6.5 and 25°C]

General description  Isoelectric point (pI): 4.7-5
pH optimum is 6-7

solubility
0.02 M phosphate, pH 6.5: 5mg/mL, clear, slightly brown to deep brown
storage temp.
?20°C


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