Glyko® PNGase F (Chryseobacterium [Flavobacterium] meningosepticum)
Specificity:
PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.
Applications:
release of intact N-linked glycans from glycopeptides and glycoproteins
structure-function studies of N-glycosylated glycoproteins
preparation of deglycosylated proteins for molecular weight estimation or crystallography studies