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CURRICULUM VITAE
PERSONAL DATA
Name: Kun-Liang Guan
Date of Birth: 4-28-63
Social Security No.: 312-90-4913
Home address: 3118 Fairhaven Ct.
Ann Arbor, MI 48105
E-mail: kunliang@umich.edu
EDUCATION:
1978-1982 Undergraduate student in the Department of Biology.
Hangzhou University, China, B.S.
1982-1983 Graduate student in Shanghai Institute of Plant
 hysiology, Shanghai, China
1983-1989 Graduate student in the Department of Biochemistry,
urdue University, West Lafayette, Indiana, Ph.D (Dec.17, 89)
(Biochemistry, Mentor: Henry Weiner)
POSTDOCTORAL TRAINING
1989-1991 ost doctoral fellow in the Department of Biochemistry,
urdue University (Mentor, Jack Dixon)
ACADEMIC APPOINTMENTS
1992-1996 Assistant Professor, Department of Biological Chemistry
and Assistant Research Scientist, Institute of Gerontology,
University of Michigan
1996-2000 Associate professor, Department of Biological Chemistry
and Aassociate Research Scientist, Institute of Gerontology,
University of Michigan
2000- rofessor, Department of Biological Chemistry, University of Michigan
CONSULTING POSITIONS
Bayer Pharmaceuticals
PATENT
DNA encoding an 18 kD cdk6 inhibitor protein, Ser. No. 08/482,069
SCIENTIFIC ACTIVITY
Panel member of the NSF cell biology study section, 1995-1999
Editorial board member, Journal of Biological Chemistry, 1997-
Editorial board member, Biological Signals and Receptors. 1999-
NIH Study Section, Molecular Biology (Ad Hoc), 1998
NIH Study Section, CDF-3, 1999-2003
GRANT SUPPORT
Past
1. University of Michigan Geriatrics Center - Regulation of Protein Tyrosine Phosphatases in Yeast, $25,000, [7/1/92 - 6/30/93], K. Guan, P.I.
2. American Cancer Society - Regulation of Protein Tyrosine Phosphorylation, 7/1/93-6/30/96, $100,000 for last years, [7/1/93-6/30/96], K. Guan, P.I.
3. University of Michigan G.I. Peptide Center - Regulation of Protein Phosphorylation/Dephosphorylation in Growth Hormone Response, 9/1/93-8/31/95, $60,000, [9/1/93-8/31/96], K. Guan, co-P.I.
4. University of Michigan Geriatrics Center - Activation of the Protein Kinase Cascade in Mitogenic Growth Hormone Response. $25,000, [7/1/93-6/30/94], K. Guan, P.I.
5. ark-Davis/Warner Lambert - The Regulation of Mitogen Activated Protein Kinases in Cellular Growth and Differentiation. $30,000, K. Guan, Co-P.I.
6. Tasho Excellency in Research Program, $71,000 (direct cost for the last year), P.I.
7. National Institute of Health-Protein Phosphorylation and Growth Factor Function, $165,000 (direct cost for the last year,1994-1998), P.I.
8. National Institute of Health-Protein kinase in T-cell activation, $31,200 (for the last year, 1997-1998), P.I.
9. arke-Davis/Warner Lambert-Function of the KSR kinase in signal transduction, $30,000 for last year, Co-P.I.
Current
Apr. 1997-Mar. 2001 GM89570-National Institute of Health-Regulation of protein tyrosine phosphorylation, $123,974 (direct cost for current year), P.I.
Aug. 1998-July 2002 GM51586-National Institute of Health-Protein Phosphorylation and Growth Factor Function, $196,344 (direct cost for current year), P.I.
Jan. 2000-June 2002 RPG-98-352-American Cancer Society, The functions of Smads in TGFb signal transduction, $80,000 (direct cost annually), P.I.
July 2000-June 2004 GM62694-National Institute of Health-Signal transduction of the Ras family small GTPases, $190,000 (direct cost for current year), P.I.
2000-2001 Regulation of the Raf kinase, Walther Cancer Institute, $105,000 (direct cost), P.I.
CERTIFICATION AND LICENSURE
None
MILITARY SERVICE
None
HONORS AND AWARDS
May 1989 The Arnold Kent Balls Award, Department of
Biochemistry, Purdue University
March 1984 Membership of The Honor Society of Agriculture,
Gamma Sigma Delta
1998-2003 MacArthur Fellow, The John D. Catherine T. MacArthur Foundation
1999 Scherling-Plaugh Award, American Society of Biochemitry and Molecular Biology
1999 Yong Investigator Award, Society of Chinese Bioscientist Association
April 19, 1999 rocter & Gamble Lecture, Purdue University
MEMBERSHIP AND OFFICES IN PROFESSIONAL SOCIETIES
AAAS
ASBMB
ASCB
TEACHING ACTIVITY
1984-1985 urdue University, Teaching assistant for
Biochemistry 207
1993- Biochemistry 576, Signal transduction, Course director
1995- Biochemistry 585, Cell cycle, Course director
ostdocs/Investigators/Lecturers Source of Support
Current trainee
Weiquan Li, Research Fellow NIH
Tomas Lanigan, Research Fellow NIH
Bao-Hong Zhang Australia Government Fellowship
Past trainee
Chaofeng Zheng, Research Fellow (1992-1994), Scientist, Stratagene
Steve Dudas, Research Fellow (1993-1995), Scientist, Henry Ford Hospital
Elizabeth Butch, Research Fellow (1993-1998), Assistant Professor, Eastern Michigan University
Toshiro Sugimoto, Research Fellow (1995-1997), Lecturer, Shiga University, Japan
Lijyun Syu, Research Fellow (1994-1996), Scientist, Parke-Davis
Reiner Fischer (1998-1999)
Seong Noh, MSTP Student
Scott Stewart, Student
Xiaoli Zhan, Student
Graduate Students:
Huira Chong, Graduate Student NIH
Jeeyong Lee, Graduate Student NIH
Eric Tang, MSTP Student NIH
Haris Vikis, Graduate Student NIH
Thesis Committee
M. Hull, Ph.D. 1993
K. Quadnt, Ph.D. 1994
M. Szczypka, Ph.D. 1995
Jirundon Yuvaniyama, Ph.D. 1997
Hangjong Duan, Ph.D. 1997
Deb Meyers, Ph.D. 1997
Larry Adams, Ph.D. 1998
Mary Stefa, Ph.D. 1999
J. Clemens, Ph.D. 1999
C. Zimmerman, Ph.D. 1999
Witoon Titrasophon, Ph.D. 1999
Sean Collin, Ph.D. 1999
X. Liu
Matt Wishart
EXTRAMURAL INVITED PRESENTATIONS (2000-)
National Institutes of Health, Feb, 2000
FASEB Summer Conference, July, 2000
Institute of Radiation Medicine, Beijing, August, 2000
Cleveland Clinic Foundation, Oct. 2000
Shiga University, Japan, Nov. 2000
Burhnam Institute, Jan, 2001
Scripps Institute, Jan. 2001
Emory University, Feb. 2001
ASBMB Annual Meeting, Orlando, Mar. 2001
Gordon Research Conference, June 2001
Peking University, China, June 2001
Institute of Molecular and Cell Biology, Singapore, July, 2001
University of Texas Medical branch at San Antonio, Nov. 2001
Cornell University, Nov. 2001
Rosewell Cancer Center, Dec. 2001
University of Texas Medical Center at Galveston, Dec. 2001
Yale University, Dec. 2001
Medical College of Ohio, Dec. 2001
Keystone Symposium, Feb.2002
Ohio State University, Mar. 2002
COMMITTEE AND ADMINISTRATIVE SERVICE (the past three years)
1995- Department Graduate Admission Committee (currently, co-chair of the department recruiting committee)
1996- Review Committee for Rackham
1998- PIBS admission committee
1994-1998 Department retreat committee
1998 Departmental internal review committee
BIBLIOGRAPHY
Completed Publication in Scientific Journals
Peer reviewed publications
1. Farres, J., Guan, K-L., and Weiner, H. (1988) Signal peptide sequence of rat mitochondrial aldehyde dehydrogenase deduced from cDNA. Biochem. Biophys. Res. Comm. 150, 1083-1087
2. Guan, K-L., Pak, Y.K., Tu, G.C., Cao, Q.N., and Weiner, H. (1988) Purification and characterization of beef and pig liver aldehyde dehydrogenases. Alcoholism: Clin. Exp. Res. 12, 713-719
3. Farres, J., Guan, K-L., and Weiner, H. (1989) Primary structures of rat and bovine liver mitochondrial aldehyde dehydrogenase deduced from cDNA sequences. Eur. J. Biochem. 180, 67-74
4. Guan, K-L., and Weiner, H. (1989) Influence of the 5’-end region of aldehyde dehydrogenase mRNA on translational efficiency: potential secondary structure inhibition of translation in vitro. J. Biol. Chem. 264, 17764-17769.
5. Guan, K-L., and Weiner, H. (1990) Sequence of the precursor of bovine liver mitochondrial aldehyde dehydrogenase as determined from its cDNA and its gene and its functionality. Arch. Biophys. Biochem. 277, 351-360.
6 Guan, K-L., Haun, R.S., Watson, S.J., Geahlen, R.L., and Dixon, J.E. (1990) Cloning and expression of a protein-tyrosine-phosphatase. Proc. Natl. Acad. Sci. USA 87, 1501-1505.
7. Guan, K-L. and Dixon, J.E. (1990) Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia. Science 249, 553-556.
8. Guan, K-L., and Dixon, J.E. (1991) One step purification of eukaryotic proteins expressed in E. coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione-S-transferase. Anal. Biochem. 192, 262-267.
9. Bliska, J.B., Guan, K-L., Dixon, J.E., and Falkow, S.A. (1991) Tyrosine phosphate hydrolysis of proteins by an essential Yersinia virulence determinant. Proc. Natl. Acad. Sci. USA 88, 1187-1191.
10. Guan, K-L., Broyles, S.S., and Dixon, J.E. (1991) Vaccinia virus encodes a Tyr/Ser protein phosphatase. Nature 350, 359-362.
11. Guan, K-L., Deschenes, R.J., Qiu, H., Dixon, J.E. (1991) Cloning and expression of a yeast protein tyrosine phosphatase. J. Biol. Chem. 266, 12964-12970.
12. Guan, K-L. and Dixon, J.E. (1991) Evidence for protein tyrosine phosphatase catalysis proceeding Via a cysteine-phosphate intermediate. J. Biol. Chem. 266, 17026-17030.
13. Ni, L.-Y., Guan, K-L., Zalkin, H., and Dixon, J.E. (1991) De novo purine nucleotide biosynthesis: cloning and expression of a eukaryotic cDNA encoding 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase. Gene 106, 197-205.
14. Guan, K-L., Deschenes, R.J., and Dixon, J.E. (1992) Isolation and characterization of a second protein tyrosine phosphatase gene, PTP2, from Saccharomyces cerevisiae. J. Biol. Chem. 267, 10024-10020
15. Guan, K-L., Hakes, D., Wang, Y., Park, H.D., Cooper, T.G., and Dixon, J.E. (1992) A novel yeast protein phosphatase related to the vaccinia virus VH1 phosphatase is induced by nitrogen starvation. Proc. Natl. Acad. Sci. USA, 89, 12175-12179.
16. Millar, J.B.A., Russell, P., Dixon, J.E., and Guan, K-L. (1992) Negative regulation of mitosis by functionally overlapping PTPases in fission yeast. The EMBO J., 11, 4943-4952.
17. Guan, K-L., Hakes, D., Park, H.D., Cooper, T.G., and Dixon, J.E. (1993). A eukaryotic homolog to viral Ser/Thr/Tyr phosphatase. Treds in Biochem. Sci. 18, 6.
18. Zheng, C.F. and Guan, K-L. (1993) Cloning and characterization of two distinct human ERK activator kinases, MEK1 and MEK2, J. Biol. Chem. 268, 11435-11439.
19. Zheng, C.F. and Guan, K-L. (1993) Dephosphorylation and inactivation of the mitogen activated protein kinases by a mitogen induced protein phosphatase. J. Biol. Chem. 268, 16116-16119.
20. Guan, K-L., Farh, L., Marshall, T.K., and Deschenes, R.J. (1993) Normal mitochondrial structure and genome maintenance in yeast requires the dynamine like product of the MGM1 gene. Current Genetics 24, 141-148.
21. Zheng, C.F. and Guan, K-L. (1993) Properties of MEKs, the kinases that phosphorylate and activate the extracellular signal-regulated kinases. J. Biol. Chem. 268, 23933-23939.
22. Zheng, C.F. and Guan, K-L. (1994) Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. The EMBO J. 13, 1123- 1131.
23. Zheng, C.F., Ohmichi, M., Saltiel, A.R., and Guan, K-L. (1994) Growth factor induced MEK activation is primarily mediated by an activator different from c-raf. Biochemistry, 33, 5595-5599.
24. Zheng, C.F. and Guan, K-L. (1994) Cytoplasmic localization of the mitogen activated protein kinase activator, MEK. J. Biol. Chem. 269, 19947-19952.
25. Frost, J.A., Alberts, A.S., Sontag, E., Guan, K-L., Mumby, M.C., and Feraminsco, J.R. (1994) Simian virus 40 small T antigen cooperates with mitogen activated kinases to stimulate AP-1 activity. Mol. Cell. Biol. 14, 6244-6252.
26. Guan, K-L., Jenkins, C.W., Li, Y., Nichols, M.A., Wu, X., Matera, G., and Xiong, Y. (1994) Growth suppression by p18, a novel p16INK4/MTS1 and p14INK/MTS2-related CDK6 inhibitor, correlates with wild type pRb function. Genes & Dev. 8, 2939-2952.
27. Guan, K-L. and Butch, E. (1995) Isolation and characterization of a novel dual specific phosphatase, HVH2, which selectively dephosphorylates the mitogen activated protein kinase. J. Biol. Chem. 270, 7197-7203.
28. Pang, R., Zheng, C.-F., Guan, K-L., and Saltiel, A.R. (1995) Nerve growth factor stimulates a novel protein kinase in PC-12 cells that phosphorylates and activates MEK. Biochem. J. 307, 513-519.
29. Pham, C.D., Arlinghaus, R.B., Zheng, C.-F., Guan, K-L., and Singh, B. (1995) Characterization of MEK1 phosphorylation by v-Mos protein. Oncogene 10, 1683-1688.
30. Wu, Y., Han, M. and Guan, K-L. (1995) Mek-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other development events. Genes & Dev. 9, 742-755.
31. Kornfeld, K., Guan, K-L., and Horvitz, H.R. (1995) The C. elegans mek-2 is required for vulval induction and encodes a protein similar to the protein kinase MEK. Genes & Dev. 9, 756-768.
32. Rui, D., Zheng, C.-F., Guan, K-L., and Williams, J. A. (1995) Activation of MAP kinase kinase (MEK) and Ras by cholecystokinin in rat pancreatic acini. Am. J. Physiol. 268, G1060-1065.
33. Wang, Q.M, Guan, K-L., Roach, P.J., and Depaoli-Roach, A.A. Phosphorylation and activation of the ATP-Mg-Dependent Protein Phosphatase by the Mitogen Activated Protein Kinase. J. Biol. Chem.270, 18352-18358.
34. Zhang, Z. Y., Zhou, G, Denu, J. M., Wu, L., Tang, X., Mondesert, O., Russell, P., Butch, E., and Guan, K-L. (1995) Purification and characterization of the low molecular weight protein tyrosine phosphatase, Stp1, from fission yeast S. pombe. Biochemistry 34, 10560-10568.
35. Chaurch, D.L., Guan, K-L. and Lambie, E.J. (1995) mek-2, mpk-1/sur-1 and let-60 ras are required for meiotic cell cycle progression in C. elegans. Development. 121, 2525-2535.
36. Waters, S. B., Holt, K.H., Ross, S.E., Syu, L.J., Guan, K-L., Saltiel, A.R., Koretzky, G.A., and Pessin, J.E. (1995) Desensitization of Ras activation by a feedback disassociation of the SOS-Grb2 complex. J. Biol. Chem. 270, 20883-20886.
37. Guan, K-L., Jenkins, C.W., Li, Y., Zariwala, M., Noh, S., Wu, X., and Xiong, Y. (1996) Molecular cloning of the CDK6 inhibitor p20 and demonstration of two distinct mechanismd of interaction with CDK proteins for two families of CDK inhibitors. Mol. Biol. Cell. 7, 57-70.
38. Sugimoto,, T., Haneda, M., Togawa, M., Isono, M., Shikano, T., Araki, S.I., Nakahiko, T., Kashiwagi, S., Guan, K-L., and Kikkawa, R. (1996) Atrial natriuretic peptide induces the expression of MKP-1, a mitogen activated protein kinase phosphatase, in glomerular mesangial cells. J. Biol. 271, 544-547.
39. Wu, X., Noh, S., Zhou, G., Dixon, J.E., Guan, K-L. (1996) Selective activation of MEK-1 but not MEK-2 by A-raf from epidermal growth factor stimulated Hela cells. J. Biol. Chem. 271, 3265-3271.
40. Butch, E. and Guan, K-L. (1996) Characterization of ERK1 activation site mutants and the effect on recognition by MEK1 and EMK2. J. Biol. Chem. 4230-4235.
41. Robinson, M.J., Cheng, M., Khokhlatchev, A., Ebert, D., Ahn, N., Guan, K-L., Stein, B., Goldsmith, E., and Cobb, M.H. (1996) Contributions of the mitogen-activated protein (MAP) kinase backbone and phosphorylation loop to MEK specificity.: J Biol Chem 271, 29734-29739.
42. Gupta, A.K., Lee, Y.L., Galoforo, S.S., Berns, C.M., Martinez, A.A., Corry, P.M., Wu, X., and Guan, K-L. Differential effect of glucose deprivation on MAPK activation in drug sensitive human breast carcinoma MCF-7 and multidrug resistant MCF-7/ADR cells. Mol. Cell. Biochem. 170, 23-30, 1997.
43. Gupta, A. K., Galoforo, S. S., Berns, C. M., Martinez, A. A., Corry, P. M., Guan, K-L., and Lee, Y. J. (1996) Elevated levels of ERK2 in human breast carcinoma MCF-7 cells transfected with protein kinase C. Cell Prolif. 29, 655-663.
44. Jiang, Y., Li, Z., Scharz, E.M., Guan, K-L., Ulevitch, R.J., and Han, J. (1997) Structure-function studies of p38 MAP kinase: loop12 influences substrate specificity and autophosphorylation but not upstream kinase selection. J. Biol. Chem. 272, 11096-1102.
45. Gorgas, G., Butch, E., Guan, K-L., and Miller, R.A. (1997) Diminished activation of the MAP kinase Pathway in CD3-stimulated T lymphocytes from old mice. Mechanism of Ageing and Dev. 94, 71-83.
46. Zhan, X., Deschenes, R.J., and Guan, K-L. (1997) Differential regulation of FUS3 MAP kinase by tryosine specific phosphatases PTP2/PTP3 and dual specificity phosphatase MSG5 in S. cerevisiae. Genes & Dev. 11, 1690-1702.
47. Syu, L-J., Guan, K-L., and Saltiel, A. R. (1997) Inducible expression of a mutant form of MEK1 in Swiss3T3 cells. J. Cell. Biochem. 67, 367-377.
48. Dabrowski, A., Groblewski, G. E., Schaefer, C., Guan, K.-L., and Williams, J.A. (1997) Cholecystokinin activates a MAP kinase cascade by mutiple mechanisms in rat acinar cells. Am. J. Physiol. 273, C1472-1479.
49. Tang, D.G., Guan, K-L., Li, L., Honn, K.V., Chen, Y.Q., Rice, R.L., Taylor, J.D., and Porter, A.T. (1997) Suppression of W256 carcinosarcoma cell apoptosis by arachidonic acid and other polyunsaturated fatty acids. Int. J. Cancer 72, 1078-1087.
50. VanderKuur, J. A., Butch, E., Waters, S.B., Pessin, J. E., Guan, K.-L., and Carter-Su, C. (1997) Signaling molecules involved in coupling growth hormone receptor to MAP kinase activation. Endocrinology 138, 4301-4307.
51. Sugimoto, T., Scott, S., and Guan, K.-L. (1997) The calcium/calmodulin dependent protein phosphatase calcineurin is the major Elk-1 phosphatase. J. Biol. Chem. 272, 29415-29418.
52. Zhao, Y., Wu, L., Noh, S. J Guan, K.-L., and Zhang, Z-Y. (1998) Altering the nucleophile specificity of a protein-tyrosine phosphatase catalyzed reaction: probing the function of the invariant glutamine residues. J. Biol. Chem. 273, 5484-5492.
53. Sugimoto, T., Scott, S., and Guan, K.-L. (1998) The kinase supressor of Ras (KSR) modulates growth factor and Ras signaling by uncoupling Elk-1 phosphorylation from MAP kinase activation. The EMBO J. 17, 1717-1727.
54. Goldman, D., Sapru, M.K., Stewart, S., Plotkin, J., Libermann, T.A., Wasylyk, B., and Guan, K.-L. (1998) Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle. Biochem. J. 335, 267-275.
55. Hodge C, Liao JF, Stofega M, Guan, K.-L., Carter-Su C, Schwartz J (1998) Growth hormone stimulates phosphorylation and activation of Elk-1 and expression of c-fos, egr-1, and junB through activation of extracellular signal-regulated kinases 1 and 2. J. Biol. Chem. 273, 31327-31336.
56. Noh, S.J., Li, Y., Xiong, Y., and Guan, K.-L. (1999) Identification of cuntional elements of p18INK4C essential for binding and inhibition of cyclin-dependent kinase (CDK)4 and CDK6. Cancer Research 59, 558-564.
57. Tan, M., Heizmann, C.W., Guan, K-L., Schafer, B.W., & Sun, Y. (1999) Transcriptional activation of the human S100A2 promoter by wildtyep p53. FEBS Lett. 445, 265-268.
58. Tan, M., Li, S., Swaroop, M., Guan, K-L., Oberley, L.W., & Sun, Y. (1999) Transcriptional activation of the human glutathione peroxidase promoter by p53. J. Biol. Chem. 274, 12061-12066.
59. Tang, E., Nunez, G., Bar, D., and Guan, K.-L. (1999) Negative regulation of the forkhead transcription factor FKHR by AKT. J. Biol. Chem. 274, 16741-16746.
60. Stewart, S., Sundaram, M., Zhang, Y., Lee, J., Han, M., and Guan, K.-L. (1999) Kinase Suppressor of Ras (KSR) forms a multi-protein signaling complex and modulates MEK localization. Mol. Cell. Biol. 19, 5523-5534.
61. Herzog, C. R., Noh, S., Lantry, L.E., Guan, K.-L., and You, M. (1999) Cdkn2a encodes functional variation of p16ink4a but not p19ARF, which conferes selection in mouse lung tumorigenesis. Mol. Carcinogenesis 25, 92-98.
62. Zhan, X. L., and Guan, K.-L. (1999) Specific protein-protein interaction accounts for the in vivo substrate selectivity of Ptp3 towards the Fus3 MAP kinase. Genes & Development. 13, 2811-2817.
63. Xiao G. Jiang D. Thomas P. Benson MD. Guan K.-L. Karsenty G. Franceschi RT. (2000) MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor, Cbfa1. Journal of Biological Chemistry. 275, 4453-4459.
64. Tan, M., Wang, Y., Guan, K-L., and Su Y. (2000) PTGF, a novel TGF- superfamily member, is a p53 target gene that mediates p53-induced growth suppression. Proc. Natl. Acad. Sci. USA 97, 109-114.
65. Zhan, X., Hong, Y., Zhu, T., Mitchell, A.P., Deschenes, R.J., and Guan, K.-L. (2000) Essential role of tyrosine phosphorylation of the RIM11 kinase in meiosis and sporulation of Saccharomyces cerevisiae. Mol. Biol. Cell. 11, 663-676.
66. Stewart, S. and Guan, K.-L. (2000) The dominant negative Ras mutant, N17Ras, can inhibit signaling independently of Ras activation. J. Biol. Chem. 275, 8854-8862.
67. Li, W., Han, M., and Guan, K.-L. (2000) The leucine-rich repeat protein Sur-8 enhances MAP kinase activation and forms a complex with Ras and Raf. Genes & Development, 14, 895-900.
68. Guan, K.-L., Figueroa, C., Brtva, T., Zhu, T., Taylor, J., Barber, T., and Vojtek, A. (2000) Negative regulation of the serine/threonine kinase B-Raf by Akt. J. Biol. Chem. 275, 27354-27359.
69. Simon, A. R., Vikis, H.G., Fanburg, B.L., Stewart, S., Cochran, B.H., and Guan, K.-L. Regulation of STAT3 by direct binding to the small G protein Rac1. Science 290, 144-147.
70. Koga, M., Zwaal, R., Guan, K-L., Avery, L., and Ohshima, Y. (2000) A MAP kinase kinase MEK-1 in C. elegans is involved in stress responses. EMBO J. 19, 5148-5156.
71. Zhang, B. H. and Guan, K-L. Activation of B-Raf kinase requires phosphorylation of the conserved residues Thr598 and Ser601. EMBO J. 19, 5429-5439.
72. Vikis H.G., Li, W, He, Z., and Guan, K.-L. The semaphorin receptor plexin-B1 specifically interacts with active Rac in a ligand dependent manner. Proc. Natl. Acad. Sci. 97, 12457-12462.
73. Li, Y., Li, M., Xing, G., Hu, Z., Wang, Q., Dong, C., Wei, H., Fan, G., Chen, J., Yang, X., Zhao, S., Chen, H., Guan, K-L., Wu, C., Zhang, C., and He, F.C. (2000) Stimulation of the Mitogen-activated Protein Kinase Cascade and Tyrosine Phosphorylation of the Epidermal Growth Factor Receptor by Hepatopoietin . J. Biol. Chem. 275, 37443-37447.
74. Tan, M., Bian, J., Guan K-L., and Sun, Y. (2001) p53CP is p51/p63, the third member of the p53 gene family: partial purification and characterization. Carcinogenesis. 22, 295-300.
75. Chong, H., Lee, J., and Guan, K.-L. (2001) Positive and negative regulation of Raf kinase activity and function by phosphorylation. EMBO J. (in press).
Non-peer reviewed publications
1. Clemens, J.C., Guan, K-L., Bliska, J.B., Falkow, S., and Dixon, J.E. (1991) Microbial Pathogenesis and tyrosine dephosphorylation: Supprising bedfellows. Mol. Microbiol. 5, 2617-2620.
2. Woodford, T.A., Guan, K-L., and Dixon, J.E. (1991) Expression of rat PTP1 in Normal and transformed cells. Adv. Prot. Phosphatases 6, 503-524.
3. Dixon, J.E., Hakes, D., and Guan K-L. (1993) Improved vectors for expression and purification of recombinant proteins. Methods in Genetics 2, 44-53.
4. Guan, K-L. and Dixon, J.E. (1993) Bacterial and viral protein tyrosine phosphatases. Seminars in Cell Biology . 4, 389-396.
5. Guan, K-L. (1994) The mitogen activated protein kinase signal transduction pathway: from the cell surface to the nucleus. Cellular Signaling 6, 581-589.
6. Wang, Y. and Guan, K-L. (1996) Regulation and function of the mitogen activated protein kinase (MAPK) signal transduction pathway. Bio/Pharma Quaterly. 1(4), 23-26.
7. Guan, K-L. and Han, M. (1999) A G-protein signaling network mediated by an RGS protein. Genes & Development 13, 1763-1767.
10. Zhang, B. H. and Guan, K-L. (2001) Regulation of the Raf kinase by phosphorylation. Experimental Lung Res. 27, 269-295.
11. Zhan, X., Wishart, M. and Guan Nonreceptor tyrosine phosphatase in cellular signaling: Regulation of mitogen activated protein kinase. (2001) Chemical Rev. (in press).
Chapters in books:
1. Weiner, H., Wang, T., Guan, K-L., Farres, J., Pak, Y.K., Saigal, D., Cunningham, S., and Jeng, J.J. (1988) Application of Molecular Biological Techniques in Alcohol Research: Study of Aldehyde Dehydrogenase in Biomedical and Social Aspects of Alcohol and Alcoholism (Kuriyama, K., Takada, A., & Ishii, H., eds) pp. 377-380, Elsevier Science Publishers B.V.
2. Guan, K-L., and Weiner, H. (1987) Molecular biology studies of beef liver aldehyde dehydrogenase in Enzymology and Molecular Biology of Carbonyl Metabolism: aldehyde dehydrogenase, aldo-keto reductase and alcohol dehydrogenase (Weiner, H. & Flynn, T.G., eds) pp. 15-24, Alan R, Liss, Inc., New York.
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