Zeocin

Zeocin, 100mg/mL

Description:

C55H83N19O21S2Cu
MW: 1137.4

Cell culture grade.

Application:

Zeocin is a copper-chelated glycopeptide antibiotic isolated from culture broth of a Streptomyces verticillus mutant. Zeocin is a versatile, cost effective antibiotic to use for selection. This unique antibiotic shows high toxicity in mammalian cells, plants, yeasts, and bacteria. Zeocin acts by binding to DNA and cleaving it, causing cell death.

Zeocin allows the selection of cells expressing the Sh ble gene. As there is no cross resistance with other currently used animal cells markers, this antibiotic can also be used to isolate clones resistant to other selecting agents (e.g. geneticin, hygromycin). Zeocin is a glycopeptide antibiotic of the bleomycin family. It is active in vivo against most bacteria (including E. coli), eukaryotic microorganisms (i.e. yeasts), plant cells, and animal cells.

USAGE:

•        Liquid Zeocin is ready for cell culture.
•        The concentration of Zeocin required for selection of transformants is pH and salt concentration dependent. The higher the pH and the lower the salt concentration, the better is Zeocin activity.
•        Selection of common E. coli strains is achieved at 25 μg/ml zeocin in Low Salt LB Agar medium adjusted to pH 7.5
•        Selection of animal cells: 100 to 750 μg/ml depending on the cell line.
•        Selection of plant cells: 50 to 250 μg/ml depending on species and media.
•        Selection of yeasts: Depends on species and media. S. cerevisiae Sh ble transformants are selected at 50 μg/ml in YPD at pH 7.0