Tail DNA Extraction Kit

Descriptions:

The Tail DNA Extraction Kit is designed for preparing high quality genomic DNA from mouse tail.

No phenol
No chloroform
No Bio-hazard

Kit Components:

Components        Description        Volume
A        Lysing Solution        50mL
B        Salt Solution        25mL
MP023        Proteinase K         1mL
MB010.1        TE Buffer        10mL

Preparation Procedures:

Materials needed but not provided in the kit:

2-mercaptoethanol
ethanol

1.        Cut 1-3 mm of mouse tail or toe tips from mice into 1.5 mL microcentrifuge tubes.
2.        Lyse tail biopsy for 4 hours to overnight in 500ul Lysing Solution (with proteinase K and 2- mercaptoethanol).

Add fresh to aliquots of solution:
        5ul 2-mercaptoethanol (not included) per 1ml lysing solution
        20ul Proteinase K (20mg/ml) per 1ml lysing solution

3.        Incubate at 55 oC.
4.        Vortex 10 seconds. Add 250ul Salt Solution and vortex 5 seconds. Let stand 15 minutes on ice.
5.        Spin 15 min at maximum speed at 4 oC
6.        Transfer supernatant to clean tube and precipitate DNA with 750ul ethanol. Mix well. Pellet by spinning gently at room temperature at speed “10” for 2-3 minutes.
7.        Wash DNA pellet with 750ul 70% ethanol and air dry. Dissolve in 100ul TE or DNase-free water. Resuspend DNA rapidly by incubating 15 minutes at 55 oC