[推荐]活体动物体内成像技术文献下载区(PDF)

精诺真体内可见光成像技术文献精选

1.  细胞凋亡与白血病
Activation of Apoptosis in Vivo by a Hydrocarbon-Stapled BH3 Helix
SCIENCE 2004，305:1466-1470
通过对BCL-2蛋白家族BID的BH3结构域进行化学修饰，使其容易穿过细胞膜，在活体内研究其激活血癌细胞发生凋亡的功能和机制。

2.  肿瘤发生
Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB
NATURE 2004，430:1034-1040
利用荧光素酶基因标记的癌细胞，研究神经营养性受体TrkB抑制细胞凋亡和促进肿瘤发生和转移的作用。

3.  肿瘤转移
Distinct organ-specific metastatic potential of individual breast cancer cells and primary tumors
THE JOURNAL OF CLINICAL INVESTIGATION 2005，115(1):44-55
观察荧光素酶标记的乳腺癌细胞在动物体内的转移情况，说明肿瘤细胞的器官特异性转移与特定系列的基因表达相关。

4.  自发肝癌
MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancer
NATURE 2004, 431:1034-1040
利用条件转基因动物，研究了致癌基因MYC在小鼠自发性肝癌形成过程中的作用。

5.  病毒载体基因治疗
Systemic tumor targeting and killing by Sindbis viral vectors
NATURE BIOTECHNOLOGY 2004, 22(1):70-77
本文依据Sindbis病毒对癌细胞表面超量表达的LAMR的识别的机理，以荧光素酶基因分别标记病毒和癌细胞, 观察标记的病毒在体内对癌细胞的靶向识别和特异性杀伤。

6.  免疫治疗
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging
BLOOD 2003， 101(2):640-648
示踪CIK细胞对肿瘤细胞的识别和清除。

7.  细胞凋亡
Non-invasive real time imaging of apoptosis
PNAS 2004, 99(26):16551-16555
在荧光素酶的两边通过连接肽接上抑制其发光的蛋白(雌激素受体), 连接肽具有Caspase-3的特异识别位点。细胞发生凋亡时, Caspase-3被激活, 切断了连接荧光素酶与其抑制蛋白间的连接肽, 恢复了荧光素酶的活性。

8.  siRNA研究
RNA interference in adult mice
NATURE   2002, 418:38-39
本文观察了人工合成的siRNA在活体动物体内阻断荧光素酶基因表达的情况，并证明通过质粒DNA模板体内转录合成的小发夹形式RNA（shRNA）可用来有效地阻断 HCV(丙肝病毒)相关基因的体内表达。

9.  干细胞
Dynamic tracking of human hematopoietic stem cell engraftment using in vivo bioluminescence imaging
BLOOD, 2003 102(10): 3478-3482
研究了荧光素酶转染的人造血干细胞在实验动物体内的增殖能力。

10.  细菌感染
Extracellular replication of Listeria monocytogenes in the murine gall bladder.
SCIENCE 2004, 303(5659):851-853
利用细菌荧光素酶基因标记的单核细胞增多性李斯特氏菌,发现其可在小鼠胆囊腔内进行胞外繁殖。

11.  病毒感染
Bioluminescence Imaging Reveals Systemic Dissemination of Herpes Simplex Virus Type 1 in the Absence of Interferon Receptors.
JOURNAL OF VIROLOGY 2003, 77(20):11082-11093
观察小鼠干扰素受体缺失情况对Ⅰ型单纯疱疹病毒（HSV-1）感染模式的影响。

12.  蛋白质相互作用
Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies
PNAS  2002, 99(24):15608-15613
在活体动物体内观察蛋白质之间的相互作用。

13.  基因表达
Tracking angiogenesis induced by skin wounding and contact hypersensitivity using a Vegfr2-luciferase transgenic mouse
BLOOD 2004, 103(2): 617-626
利用表达荧光素酶的转基因动物研究特定基因的时空表达情况。

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精诺真活体动物体内成像技术在药物研究领域的文献精选

1．  Cdk2抑制剂的药物动力学模型
Bioluminescent imaging of Cdk2 inhibition in vivo
Nature Medicine 10, 643-648, Jun 2004

2．  喜树碱类抗癌新药托泊替康(topotecan)的临床前研究
Schedule-dependent inhibition of hypoxia-inducible factor –1α protein accumulation ,angiogenesis ,and tumer growth by topotecan in U251 HRE glioblastoma xenografts
Cancer Research 64, 6845-6848, Oct 1, 2004

3．  Sugen公司(已经被Pfizer收购)抗乳腺癌药物SU11248的临床前药效评价
In Vivo Antitumer Activity of SU11248，a Novel Tyrosine Kinase Inhibitor Targeting Vascular Endothelial Growth Factor and Platelet-derived Growth Factor Receptors: Determination of a Pharmacokinetic/Pharmacodynamic Relationship
Clinical Cancer Research 9, 327-337, Jan 2003

4．  抑制低氧诱导因子通路的小分子药物黑毛霉素（chetomin）抗癌疗效评价
Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway
Cancer cell , 6 ,33-43 ,Jul ,2004

5．  酪氨酸激酶抑制剂PKC412治疗急性淋巴细胞白血病的疗效观察
Inbibition of FLT3 in MLL:Validation of a therapeutic target identified by gene expression based classification
Cancer cell , 3,173-183 ,Feb ,2003

6．  AMD 3100 治疗原发性脑肿瘤的药效评价
A small-molecule antagonist of CXCR4 inhibits intracranial growth of primary brain tumors.
PNAS 100(23), 13513-13518, Nov 11, 2003

7．  MAP激酶抑制剂CI 1040治疗黑色素瘤肺转移的疗效观察
Treatment of Metastatic Melanoma with an Orally Available Inhibitor of the Ras-Raf-MAPK Cascade.
Cancer Research 63, 5669-5673, Sep 15, 2003

8．  细胞毒抗癌药物MLN2704（抗体化疗复合物）治疗成骨细胞前列腺癌转移的疗效观察
A Prostate-Specific Membrane Antigen-Targeted Monoclonal Antibody-Chemotherapeutic Conjugate Designed for the Treatment of Prostate Cancer.
Cancer Research 64, 7995-8001, Nov 1, 2004

9．  Epothilone 类似物治疗骨髓瘤的疗效观察
Investigation of antitumor effects of synthetic epothilone analogs in human myeloma models in vitro and in vivo
PNAS 102(30): 10640-10645 , July 26 , 2005

10．  药物代谢
The orphan nuclear receptor HNF4α determines PXR- and CAR-mediated xenobiotic induction of CYP3A4.
Nature Medicine 9(2), 220-224, Feb 2003

11．  药物抗药性逆转筛选模型
Imaging reversal of multidrug resistanse in living mice with bioluminescence : MDR1 P-glycoprotein transports coelenterazine
PNAS 101,1702-1707 ,Feb 10 ,2004

12．  药物对蛋白质相互作用的影响
Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals
PNAS 101(33), 12288-12293, Aug 17 , 2004

13．  治疗传染性心内膜炎的抗生素筛选模型
Real-time in vivo bioluminescent imaging for evaluating the efficacy of antibiotics in a rat Staphyococcus aureus endocarditis model
Antimicrobial Agent and Chemotherapy 49(1): 380–387，Jan , 2005

14．  抗炎症化合物药物筛选模型
An Inducible Nitric Oxide Synthase-Luciferase Reporter System for In Vivo Testing of Anti-inflammatory Compounds in Transgenic Mice.
The Journal of Immunology, 2003, 170: 6307–631

15．  药物毒理评价模型
In vivo bioluminescent monitoring of chemical toxicity using heme oxygenase-luciferase transgenic mice
Toxicology and Applied Pharmacology 200(3):219-28 ， Nov 1 ，2004

16．  关于药物研究动物模型的一篇综述
Reporter mice and drug discovery and development
Nature Reviews Drug Discovery Vol. 4, 249-255, March 2005

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