深入了解：微囊藻毒素

kanyinbi · 发表于 2006-1-20 17:12:00

微囊藻毒素系列：fficeffice" />

Microcystin-LR

规格：100µg ， 500µg ， 1mg

Microcystin-RR

规格：250µg

Microcystin-LW

规格：25µg

Microcystin-LF

规格：25µg

Microcystin-YR

规格：25µg

Product Specifications

FORMULA: C52H72N10O13

MW: 1045.2

CAS NUMBER: 101064-48-6

SOURCE/HOST: Isolated from Microcystis aeruginosa.

PURITY: ³95%

IDENTITY: Identity determined by MS.

APPEARANCE: Whitish film adhered to inside of the vial.

SOLUBILITY: Soluble in 100% ethanol, methanol or DMSO.

SHIPPING: AMBIENT

LONG TERM STORAGE: ffice:smarttags" />-20°C

HAZARD: HIGHLY IRRITANT. VERY TOXIC. TUMOR PROMOTER.

Product Description

Analog of microcystin-LR (Prod. No. ALX-350-012) with Tyr substituted in place of Leu. As for all

microcystins the conjugated double bonds in the ADDA moiety cause a characteristic absorption

maximum at 238nm. The Tyr residue in position 2 of microcystin-YR confers an absorption maximum at

232nm. Potent inhibitor of eukaryotic protein phosphates 1 and 2A. Useful as a reference compound in

environmental analysis. The hydroxyl group of the Tyr residue may prove useful for linking microcystin-YR

via conjugation to other chemicals.

Monoclonal Antibody to Microcystin-LR (MC10E7)

规格：200 μg

Product Specifications

CLONE: MC10E7

ISOTYPE: Mouse IgG1

IMMUNOGEN: Microcystin-LR linked via N-methyl-dehydroalanine to cationized

ovalbumin.

SPECIFICITY: Recognizes all 4-Arg microcystins.

APPLICATION: ELISA (1:10'000-1:100'000)

Microcystin concentrations can be measured in the range of

~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1'000 times

higher compared to UV detection.

FORMULATION: Lyophilized from antibody purified by protein G affinity

chromatography.

RECONSTITUTION: Reconstitute with 200μl PBS containing sodium azide.

SHIPPING: SHIPPED ON BLUE ICE

LONG TERM STORAGE: -20°C

USE/STABILITY: Storage at 4°C will decrease antibody activity and must be avoided.

HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store

at -20°C.

Product Description

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50

values for microcystin-LR of 0.06 μg/l have been obtained. The provisional guideline value proposed by

the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin

variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other

microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was

determined to be at least 7 x 1010 l/mol.

The antibody was tested for its robustness against interferences (humic acids, pH, salt content,

surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in

the concentration range between 0.01 and 0.1 μg/l were measured and a mean recovery of 99.9 ± 16.4%

was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as

surface water.

Monoclonal Antibody to Microcystins (Addaspecific) (AD4G2)

规格：100 μg

Product Specifications

CLONE: AD4G2

ISOTYPE: Mouse IgG1

IMMUNOGEN: N-Acetyl-Adda-KLH and N-Acetyl-Adda-BSA.

SPECIFICITY: Recognizes all microcystins.

APPLICATION: ELISA (1:10'000)

Microcystins can be measured at a detection limit of ~0.03μg/l.

FORMULATION: Lyophilized.

RECONSTITUTION: Reconstitute with 100μl PBS containing sodium azide.

SHIPPING: SHIPPED ON BLUE ICE

SHORT TERM STORAGE: +4°C

LONG TERM STORAGE: -20°C

HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid

freeze/thaw cycles.

POSITIVE CONTROL: Products No. ALX-350-012, ALX-350-043, ALX-350-080 and

ALX-350-081.

Product Description

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody AD4G2, IC50

values for microcystin-LR of 0.06 μg/l have been obtained. The provisional guideline value proposed by

the WHO is 1μg/l for drinking water. The detection limit for microcystin-LR is 0.07μg/l. All microcystin

variants show similar IC50 values and detection limits. No microcystins are known, that are not recognized

by this antibody. Microcystin-LR spiked water samples in the concentration range between 0.1 and 1μg/l

were measured and a mean recovery of 113 ± 23% was found. The antibody is well suited for the

sensitive analysis of all microcystins in drinking as well as surface water. Due to the very even

cross-reactivity pattern of the antibody, a calibration with one microcystin (preferably MC-LR) (Prod. No.

ALX-350-012) is sufficient for the quantitative determination of a sum concentration of all microcystins

contained in a a sample.

</DIV>

Nodularin

规格：10ug , 100ug , 250ug

Product Specifications

FORMULA: C41H60N8O10

MW: 825.0

CAS NUMBER: 118399-22-7

SOURCE/HOST: Isolated from Nodularia spumigena.

PURITY: ³96%

APPEARANCE: Dry residue containing traces of monobasic potassium phosphate.

SOLUBILITY: Soluble in methanol:water (1:1).

SHIPPING: AMBIENT

LONG TERM STORAGE: -20°C

HAZARD: TUMOR PROMOTER. HIGHLY IRRITANT.

Product Description

Inhibitor of protein phosphatase 1 (PP1) (IC50=1.8nM), protein phosphatase 2A (PP2A) (IC50

=0.026nM) and to a lesser extent protein phosphatase 2B (PP2B) (IC50=8.7μm). Similar to microcystin-LR

(Prod. No. ALX-350-012) but with increased water solubility.

</DIV>

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