[转贴]前脂肪细胞培养基突破！！！

New and Improved Preadipocyte Growth Media Kit (PGM-2)

For subcutaneous and visceral preadipocyte cell culture

Visceral Preadipocyte Cell System

Product Specifications
Part Description Size
PT-8002 PGM-2 Bullet Kit KIT
PT-8202 PBM-2 (Basal Medium) 500 ml
PT-9502 PGM-2 SingleQuots® Kit KIT

Shipping Conditions
Koolits and Dry Ice. Product not to be shipped over weekend.

Storage and Handling
Store basal medium at 4-8 °C.
Store SingleQuots at -20 °C.

Description
PoieticsTM Growth Media 2 Kit is an improved formulation for culturing preadipocytes based on our existing PGM media kit. This media kit includes the required ingredients for cell proliferation and differentiation of subcutaneous and visceral preadipocytes. This new media kit outperforms our older version and several major competitors’ media in both proliferation and differentiation.

Competing Products/Technologies
The 2 primary competitors are Promocell and ZenBio which have similar media kits for growing and differentiating preadipocytes. Promocell’s kit performed miserably with our cells repeatedly. They are also much more expensive, contain glitazones, and require longer culture time. ZenBio’s kit performs adequately but requires much longer culture times which involves additional feeding and media changes. They are also more expensive and send their media fresh so it’s shelf life is only 6 months at best (if kept frozen).

Product Position

Superior PGM-2 Media significantly outperforms competition:
Better proliferation of visceral and subcutaneous cells
Significantly better differentiation of cells in much shorter culture time (10 days)
No glitazones included which can interfere with PPAR-gamma activation assays
No media changes needed during culture time.
Longer shelf life (1 year vs. 6 months)
Easy to use, and more cost effective kit

Related Products

PT-5001 Subcutaneous Preadipocytes (4 million cells)
PT-5020 Subcutaneous Preadipocytes (1 million cells)
PT-5005 Visceral Preadipocytes (1 million cells)
PT-7009 AdipoRed Assay Reagent

Customer Questions
1. How do the media recommendations differ for the visceral vs. subcutaneous preadipocytes since the same media kit is used? There is now no difference in protocol between visceral and subcutaneous cells with this new media.
2. Have the passage numbers increased with the new media? No, 2 passages recommended for subcutaneous and 1 passage for visceral.
3. Can I still purchase PGM? PGM will be discontinued on June 30th and completely replaced by PGM-2.
4. What level of differentiation should I expect to see from these cells? We guarantee >70% of the visceral cells, and 90% of the subcutaneous will differentiate into mature adipocytes when using our recommended protocols.
5. What are the formulation differences between PGM and PGM-2? Additional component added to the basal medium, and different concentrations of 2 components in the SQ kit.