人血清一氧化氮（NO）ELISA Kit

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预期应用 ELISA法定量测定人血清、血浆、或其它相关液体中NO含量。   实验原理 本试剂盒应用双抗体夹心酶标免疫分析法测定标本中NO水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入NO抗原、生物素化的抗人NO抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的NO呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。   Intended use This immunoassay kit allows for the specific measurement of human NO concentrations in serum and plasma.   Test principle This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for NO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NO present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for NO is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NO bound in the initial step. The color development is stopped and the intensity of the color is measured.

[此贴子已经被作者于2007-5-26 9:05:19编辑过]